Enzymes
Product List
ARSA Human, SF9
Arylsulfatase A Human Recombinant, Sf9
ARSA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 498 amino acids (21-509a.a.) and having a molecular mass of 53.0kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). ARSA is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
ARSA Human
Arylsulfatase A Human Recombinant
ARSA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
ARSA Mouse
Arylsulfatase A Mouse Recombinant
ARSA Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 498 amino acids (18-506 a.a.) and having a molecular mass of 53.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). ARSA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
ARSA Mouse, Active
Arylsulfatase A Mouse Recombinant, Active
ARSA Mouse Recombinant produced in Sf9 is a single, glycosylated polypeptide chain containing 498 amino acids (18-506) and having a molecular mass of 53.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
ARSA Mouse is fused to an 9 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
ARSG Human
Arylsulfatase G Human Recombinant
ARSG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
IDS Human
Iduronate 2-Sulfatase Human Recombinant
SUMF1 Human
Sulfatase Modifying Factor 1 Human Recombinant
SUMF1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SUMF1 Human, Sf9
Sulfatase Modifying Factor 1 Human Recombinant, Sf9
SUMF1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 347 amino acids (34-374.a.) and having a molecular mass of 38.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). SUMF1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Insect cells.
Introduction
Sulfatases are a class of enzymes that catalyze the hydrolysis of sulfate esters into an alcohol and a bisulfate . These enzymes are crucial in the metabolism of sulfate esters, which are found in a variety of biological molecules including steroids, carbohydrates, and proteins . Sulfatases are classified into four main families based on sequence homology:
Sulfatases are expressed in various cellular compartments such as the lysosome, endoplasmic reticulum, and Golgi apparatus . They are activated by the unusual amino acid formylglycine, which is formed from cysteine at the catalytic center . These enzymes are extensively glycosylated and follow specific trafficking pathways through cells . Sulfatases are distributed in a wide range of tissues, including the central and peripheral nervous systems, connective tissues, liver, placenta, and skin .
Sulfatases play a pivotal role in the regulation of cell metabolism and developmental cell signaling . They cleave sulfate groups from sulfate esters contained in hormones, proteins, and complex macromolecules . This activity is essential for the homeostasis of the body, maintaining neuronal function, chondrogenesis, detoxification, steroid hormone inactivation, and skin humidification . Sulfatases are also involved in immune responses and pathogen recognition .
Sulfatases operate by hydrolyzing sulfate esters through a highly conserved cysteine in their active site, which is post-translationally converted into formylglycine . This post-translational modification activates all sulfatases . They interact with various molecules and cells, including proteoglycans, glycoproteins, and glycolipids, modulating specific signaling pathways and cell metabolism . Sulfatases also play a role in the degradation of sulfated glycosaminoglycans and glycolipids in the lysosome .
The expression and activity of sulfatases are regulated by various mechanisms, including transcriptional regulation and post-translational modifications . Cytokines, growth factors, and steroids can influence sulfatase activity . The formylglycine-generating enzyme encoded by SUMF1 is crucial for the activation of sulfatases through post-translational modification . Additionally, sulfatases are extensively glycosylated, which affects their trafficking and function within cells .
Sulfatases have significant applications in biomedical research, diagnostic tools, and therapeutic strategies . They are used in the study of metabolic disorders, lysosomal storage diseases, and hormone-dependent cancers . Sulfatases are also employed in the development of enzyme replacement therapies and gene therapies . In addition, they have potential applications in biocatalysis for the production of novel sulfated oligomers used in pharmaceuticals and cosmetics .
Sulfatases play a crucial role throughout the life cycle, from development to aging and disease . During development, they are involved in the regulation of cell signaling and metabolism . In adulthood, sulfatases maintain homeostasis and are essential for various physiological processes . In aging and disease, defects in sulfatase activity can lead to lysosomal storage disorders and other metabolic conditions . Sulfatases are also implicated in the immune response and cognitive function .
