ARSA Mouse, Active
Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
ARSA Mouse Recombinant produced in Sf9 is a single, glycosylated polypeptide chain containing 498 amino acids (18-506) and having a molecular mass of 53.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
ARSA Mouse is fused to an 9 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Recombinant ARSA Mouse protein, expressed in Sf9 cells, is a single, glycosylated polypeptide chain consisting of 498 amino acids (positions 18-506), with a molecular mass of 53.2 kDa. It's important to note that on SDS-PAGE, the apparent molecular size might range from 50 to 70 kDa. The ARSA Mouse protein includes a 9-amino acid His-Tag fused at its C-terminus and is purified using proprietary chromatographic techniques.
The ARSA protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a solution of Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73.
ADPSPPNILL IFADDLGYGD LGSYGHPSST TPNLDQLAEG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRSGMYPGVLGPS SQGGLPLEEVTLAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPD IPCKGGCDQG LVPIPLLANL TVEAQPPWLPGLEARYVSFS RDLMADAQRQ GRPFFLYYAS HHTHYPQFSG QSFTKRSGRG PFGDSLMELD GAVGALMTTV GDLGLLEETL VIFTADNGPELMRMSNGGCSGLLRCGKGTT FEGGVREPAL VYWPGHITPG VTHELASSLD LLPTLAALTG APLPNVTLDG VDISPLLLGT GKSPRKSVFFYPPYPDEIHG VFAVRNGKYK AHFFTQGSAH SDTTSDPACH AANRLTAHEP PLLYDLSQDP GENYNVLESI EGVSPEALQA LKHIQLLKAQYDAAMTFGPS QIAKGEDPAL QICCQPSCTP HPVCCHCPGS QSHHHHHH.
Product Science Overview
Arylsulfatase A (ARSA), also known as cerebroside-sulfatase, is an enzyme that hydrolyzes cerebroside sulfate into cerebroside and sulfate . In the context of recombinant proteins, Mouse Recombinant Arylsulfatase A refers to the ARSA enzyme produced using recombinant DNA technology in a mouse cell line .
ARSA plays a crucial role in the degradation of sulfatides, which are essential components of the myelin sheath in the nervous system . The enzyme’s activity is vital for maintaining the normal function of the nervous system. Deficiency in ARSA activity leads to the accumulation of sulfatides, causing a lysosomal storage disorder known as metachromatic leukodystrophy (MLD) .
The enzyme ARSA hydrolyzes the sulfate ester bond in cerebroside sulfate, converting it into cerebroside and sulfate . This reaction is essential for the catabolism of sulfatides in the lysosomes. The enzyme’s activity is measured by its ability to hydrolyze synthetic substrates such as 4-Nitrocatechol Sulfate (PNCS) .
Recombinant Mouse Arylsulfatase A is used in various research applications, including studies on lysosomal storage disorders, enzyme replacement therapies, and the biochemical pathways involving sulfatides . Its activity and stability make it a valuable tool for understanding the molecular mechanisms underlying MLD and other related diseases.
