ARSA Mouse

Arylsulfatase A (ARSA) is an enzyme that belongs to the sulfatase family. It is encoded by the ARSA gene and plays a crucial role in the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheath . The recombinant form of this enzyme, derived from mouse sources, is used in various research and therapeutic applications.

The deduced amino acid sequence of mouse ARSA consists of a signal peptide (residues 1-17) and a mature chain (residues 18-506) . The enzyme’s activity is measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS), with a specific activity of over 100 pmol/min/µg . The recombinant mouse ARSA protein is typically produced in a mouse myeloma cell line (NS0) and includes a C-terminal 10-His tag for purification purposes .

ARSA is essential for the degradation of cerebroside-3-sulfate in lysosomes. Deficiency in ARSA activity leads to a lysosomal storage disease known as metachromatic leukodystrophy (MLD), which affects the central and peripheral nervous systems and results in severe and progressive neurological symptoms . The recombinant form of ARSA is used to study the enzyme’s function and to develop potential therapies for MLD.

Recombinant mouse ARSA is used in various research applications, including enzyme activity assays, structural studies, and therapeutic research. It is supplied as a 0.2 μm filtered solution in Tris and NaCl and is stable for up to six months when stored at -20 to -70 °C .