Enzymes
Product List
CES1 Human
Carboxylesterase 1 Human Recombinant
CES1 Human produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 559 amino acids (19-568 a.a.) and having a molecular mass of 61.7kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
CES1 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
ACHE Human
Acetylcholinesterase Human Recombinant
ACHE Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (32-614 a.a) containing a total of 592 amino acids, having a molecular mass of 65.6 kDa.
ACHE is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
ACOT11 Human
Acyl-CoA Thioesterase 11 Human Recombinant
ACOT13 Human
Acyl-CoA Thioesterase 13 Human Recombinant
ACOT7 Human
Acyl-CoA Thioesterase 7 Human Recombinant
ACOT7 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
ACOT8 Human
Acyl-CoA Thioesterase 8 Human Recombinant
CES1D Mouse
Carboxylesterase 1D Mouse Recombinant
CES1D is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
CES1G Mouse
Carboxylesterase 1G Mouse Recombinant
CES1G is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Esterase D Human
Esterase D,S-Formylglutathione Hydrolase Human Recombinant
The Esterase-D is purified by proprietary chromatographic techniques.
PDE6D Human
Phosphodiesterase 6D cGMP-Specific Rod Delta Human Recombinant
Introduction
Esterases exhibit diverse biological properties, including broad substrate specificity and stability under various conditions. They are expressed in multiple tissues, including the liver, blood plasma, and various microbial environments . Their tissue distribution is extensive, with significant roles in lipid metabolism and detoxification processes .
Esterases play crucial roles in various biological functions:
Esterases interact with other molecules and cells through specific binding partners and downstream signaling cascades. The catalytic mechanism typically involves a serine residue in the active site, which attacks the carbonyl carbon of the ester bond, facilitated by histidine and aspartic/glutamic acid residues . This leads to the formation of an acyl-enzyme intermediate, which is subsequently hydrolyzed to release the products .
The expression and activity of esterases are regulated through various mechanisms:
- Transcriptional Regulation: Gene expression is controlled by transcription factors and regulatory elements in the promoter regions .
- Post-Translational Modifications: Esterase activity can be modulated by phosphorylation, glycosylation, and other modifications .
- Environmental Factors: pH, temperature, and the presence of inhibitors or activators can influence esterase activity .
Esterases have numerous applications in biomedical research, diagnostics, and therapeutics:
- Biomedical Research: Used as tools to study lipid metabolism and enzyme kinetics .
- Diagnostic Tools: Employed in assays to detect specific ester substrates or products .
- Therapeutic Strategies: Targeted for drug development, particularly in the treatment of metabolic disorders and detoxification therapies .
Throughout the life cycle, esterases play vital roles from development to aging and disease:
- Development: Involved in the regulation of lipid metabolism during growth and development .
- Aging: Changes in esterase activity are associated with aging processes and age-related diseases .
- Disease: Altered esterase activity is linked to various diseases, including metabolic disorders, neurodegenerative diseases, and cancer .
