ACOT13 Human

Acyl-CoA Thioesterase 13 Human Recombinant
Cat. No.
BT17994
Source
Escherichia Coli.
Synonyms
Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13, Thioesterase superfamily member 2, ACOT13, THEM2, HT012, MGC4961, PNAS-27, ACOT13.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACOT13 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 160 amino acids (1-140 a.a.) and having a molecular mass of 17.1kDa. The ACOT13 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Acyl-coenzyme A thioesterase 13 (ACOT13) is a member of the thioesterase subfamily within the esterase family. It is highly expressed in the kidney, with moderate expression in the brain, liver, and intestines. ACOT13 possesses a hotdog-fold structure and is believed to co-localize with microtubules, potentially playing a role in cellular proliferation. A deletion of a segment on chromosome 6's q arm is associated with early-onset intestinal cancer, suggesting the presence of a cancer susceptibility locus in that region.
Description
Recombinant human ACOT13, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 160 amino acids, including a 20 amino acid His tag at the N-terminus, spanning amino acids 1-140. The protein has a molecular mass of 17.1 kDa. Purification of ACOT13 is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
The ACOT13 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13, Thioesterase superfamily member 2, ACOT13, THEM2, HT012, MGC4961, PNAS-27, ACOT13.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN.

Product Science Overview

Biological Function and Importance

ACOT13 is a cytoplasmic protein that exists as a homotetramer and is associated with mitochondria . It has been identified as an interacting protein of phosphatidylcholine transfer protein (PC-TP), which is involved in lipid metabolism . The enzyme is broadly expressed and plays a significant role in regulating hepatic lipid and glucose metabolism .

In mice, the orthologous protein has been shown to regulate fatty acid metabolism and glucose homeostasis. For instance, mice lacking Them2 exhibit altered fatty acid metabolism and reduced hepatic glucose production . This suggests that ACOT13 is crucial for maintaining metabolic balance in the liver.

Recombinant Human ACOT13

Recombinant human ACOT13 is produced using human embryonic kidney (HEK293) cells . The recombinant protein is typically tagged with a His-tag for purification purposes and is available in a lyophilized form for research use . The recombinant protein retains the functional properties of the native enzyme, making it a valuable tool for studying lipid metabolism and related pathways.

Applications in Research

ACOT13 is used in various research applications, particularly in studies related to lipid metabolism, diabetes, and obesity. By understanding the role of ACOT13 in these processes, researchers can develop new therapeutic strategies for metabolic diseases.

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