Enzymes
Product List
DCT Human
Dopachrome Tautomerase Human Recombinant
DCT Human Recombinant produced in Sf9 Insect cell is a single, glycosylated polypeptide chain containing 455 amino acids (24-472aa.a) and having a molecular mass of 52.1kDa. DCT is fused to a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
DsbC
Disulfide-Bond Isomerase Recombinant
Disulfide-Bond Isomerase Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (21-236) and having a molecular mass of 23.6 kDa.
DsbC is purified by proprietary chromatographic techniques.
ECI1 Human
Enoyl-CoA Delta Isomerase 1 Human Recombinant
ECI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
GNPDA1 Human
Glucosamine-6-Phosphate Deaminase 1 Human Recombinant
GNPDA1 is fused to a 20 amino macid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
GPI Human
Glucose-6-Phosphate Isomerase Human Recombinant
The GPI is purified by proprietary chromatographic techniques.
GPI Human, Active
Glucose-6-Phosphate Isomerase Human Recombinant, BioActive
GPIHuman Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 578 amino acids (1-558) and having a molecular mass of 65.3 kDa.
GPI is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Escherichia Coli.
IDI1 Human
Isopentenyl-Diphosphate Delta Isomerase 1 Human Recombinant
IDI1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
IDI2 Human
Isopentenyl-Diphosphate Delta Isomerase 2 Human Recombinant
IDI2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 247 amino acids (1-227 a.a.) and having a molecular mass of 28.9kDa.
IDI2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MPI Human
Mannose Phosphate Isomerase Human Recombinant
The MPI is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
PDI Human
Protein Disulfide Isomerase Human Recombinant
PDI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing a total of 502 amino acids and having a molecular mass of 56.6kDa. The PDI is fused to a 12 amino acid His tag at N-terminal and purified by proprietary chromatographic techniques.
Introduction
Isomerases are a class of enzymes that catalyze the conversion of molecules from one isomer to another. This process involves intramolecular rearrangements where bonds are broken and formed, resulting in a product with the same molecular formula but different structural or spatial arrangements . Isomerases are classified into several subcategories based on the type of isomerization they catalyze:
- Racemases and Epimerases: Catalyze the conversion of one stereoisomer to another.
- Cis-trans Isomerases: Catalyze the conversion between cis and trans isomers.
- Intramolecular Lyases: Catalyze the breaking and forming of bonds within a molecule.
- Intramolecular Transferases: Transfer functional groups within a molecule.
- Intramolecular Oxidoreductases: Catalyze oxidation-reduction reactions within a molecule .
Isomerases exhibit diverse biological properties, including their expression patterns and tissue distribution. These enzymes are typically proteins with specific three-dimensional structures that enable their catalytic activity . The expression of isomerases can vary significantly across different tissues and developmental stages, reflecting their specialized roles in various biological processes. For example, glucose isomerase is widely distributed in bacteria, actinomycetes, fungi, and plants .
Isomerases play crucial roles in numerous biological functions. They are involved in essential metabolic pathways such as glycolysis and carbohydrate metabolism . For instance, glucose isomerase catalyzes the reversible isomerization of D-glucose and D-xylose to D-fructose and D-xylulose, respectively . Additionally, isomerases can participate in immune responses and pathogen recognition by facilitating the structural rearrangement of molecules involved in these processes .
The mechanisms of action of isomerases involve interactions with other molecules and cells. These enzymes typically bind to their substrates through specific active sites, facilitating the conversion of one isomer to another . For example, glucose isomerase binds to glucose and catalyzes its conversion to fructose through a series of intramolecular rearrangements . The downstream signaling cascades triggered by isomerase activity can vary depending on the specific enzyme and its role in the cell.
The expression and activity of isomerases are tightly regulated through various mechanisms. Transcriptional regulation involves the control of gene expression at the level of transcription, ensuring that isomerases are produced in response to specific cellular needs . Post-translational modifications, such as phosphorylation and glycosylation, can also modulate the activity and stability of isomerases . These regulatory mechanisms ensure that isomerases function optimally within the cellular environment.
Isomerases have numerous applications in biomedical research, diagnostic tools, and therapeutic strategies. For example, glucose isomerase is extensively used in the industrial production of high-fructose corn syrup and bioethanol . In biomedical research, isomerases are employed to study metabolic pathways and enzyme kinetics. Additionally, isomerases can serve as diagnostic markers for certain diseases and as targets for therapeutic interventions .
Isomerases play vital roles throughout the life cycle, from development to aging and disease. During development, isomerases are involved in key metabolic processes that support growth and differentiation . In aging, the activity of isomerases can influence cellular metabolism and the maintenance of cellular homeostasis. Dysregulation of isomerase activity has been implicated in various diseases, including metabolic disorders and cancer .
