ECI1 Human

ECI1 plays a pivotal role in the mitochondrial beta-oxidation pathway, which is essential for the degradation of fatty acids. The enzyme catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters into 2-trans-enoyl-CoA intermediates . This transformation is a key step in the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids .

The human recombinant form of ECI1 is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 284 amino acids . It has a molecular mass of approximately 31.1 kDa . The enzyme is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques .

The ECI1 protein solution is typically formulated with 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT . For optimal stability, it should be stored at 4°C if used within 2-4 weeks, or frozen at -20°C for longer periods . It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage to avoid multiple freeze-thaw cycles .

ECI1 is associated with lipid metabolism disorders and is involved in pathways related to fatty acid metabolism . Its activity is crucial for maintaining proper metabolic functions, and any disruptions in its function can lead to metabolic disorders .

Gene Ontology (GO) annotations related to ECI1 include delta(3)-delta(2)-enoyl-CoA isomerase activity and intramolecular oxidoreductase activity . The enzyme is also involved in various metabolic pathways, highlighting its importance in cellular metabolism .