DCT Human
Description
DCT Human Recombinant produced in Sf9 Insect cell is a single, glycosylated polypeptide chain containing 455 amino acids (24-472aa.a) and having a molecular mass of 52.1kDa. DCT is fused to a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Dopachrome tautomerase (DCT), also known as tyrosine-related protein 2 (TYRP2), is an enzyme involved in the melanin biosynthetic pathway. It catalyzes the conversion of dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), a key step in melanin production . This enzyme is encoded by the DCT gene located on chromosome 13 in humans .
DCT plays a crucial role in the pigmentation process by regulating the type and amount of melanin produced. Melanin is responsible for the color of skin, hair, and eyes, and provides protection against ultraviolet (UV) radiation . The enzyme’s activity is regulated by the microphthalmia-associated transcription factor (MITF), which is essential for the development and function of melanocytes .
Human recombinant DCT is produced using recombinant DNA technology, which involves inserting the human DCT gene into a suitable expression system, such as bacteria or yeast, to produce the enzyme in large quantities. This recombinant form is used in various research applications to study its function, structure, and role in diseases .
DCT has been implicated in the resistance of melanoma cells to chemotherapy and radiotherapy. Studies have shown that melanoma cells with high levels of DCT expression are more resistant to these treatments, while cells with low DCT levels are more susceptible . This suggests that DCT could be a potential target for improving the effectiveness of cancer therapies .
