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Product List
AURKA Human
Aurora Kinase A Human Recombinant
AURKA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 423 amino acids (1-403) and having a molecular mass of 47.9kDa. AURKA is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs 
Cat. No.
BT27786
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.
AURKB Human
Aurora Kinase B Human Recombinant
AURKB Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 364 amino acids (1-344) and having a molecular mass of 41.4kDa. AURKB is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27871
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.
Introduction
Definition and Classification
Aurora kinases are a family of serine/threonine kinases that play a crucial role in cell division by controlling chromatid segregation . They are essential for cell proliferation and are involved in various stages of mitosis and meiosis . There are three main types of Aurora kinases in mammals:
- Aurora A (AURKA): Functions during prophase of mitosis and is required for correct duplication and separation of centrosomes .
- Aurora B (AURKB): Functions in the attachment of the mitotic spindle to the centromere .
- Aurora C (AURKC): Works primarily in germ-line cells and is less understood compared to Aurora A and B .
Biological Properties
Aurora kinases share a similar protein structure and kinase activity but exhibit distinct cellular and subcellular localization .
- Aurora A: Promotes centrosome maturation and mitotic spindle assembly, favoring the G2/M transition .
- Aurora B: Part of the chromosome-passenger complex, crucial for chromosome binding to kinetochores and segregation .
- Aurora C: Mainly expressed in meiotically-active germ cells . In human tumors, all Aurora kinase members play oncogenic roles related to their mitotic activity and promote cancer cell survival and proliferation .
Biological Functions
Aurora kinases are essential for the onset and progression of mitosis .
- Aurora A: Involved in centrosome maturation, spindle assembly, and chromosome alignment .
- Aurora B: Ensures proper chromosome segregation and cytokinesis .
- Aurora C: Functions in germ cells, although its specific roles are less well characterized . These kinases are also implicated in immune responses and pathogen recognition through their roles in cell division and proliferation .
Modes of Action
Aurora kinases interact with various molecules and cells to execute their functions .
- Aurora A: Activated by autophosphorylation and binding to the spindle protein TPX2 . It also interacts with other proteins to regulate centrosome function and spindle assembly .
- Aurora B: Functions as part of the chromosome-passenger complex, interacting with kinetochores and microtubules to ensure proper chromosome segregation .
- Aurora C: Less understood but believed to function similarly to Aurora B in germ cells .
Regulatory Mechanisms
The activity of Aurora kinases is tightly regulated through various mechanisms .
- Phosphorylation: Key regulatory mechanism for activation, particularly for Aurora A .
- Degradation: Ensures timely inactivation of the kinases to prevent abnormal cell division .
- Transcriptional Regulation: Controlled by cell cycle-dependent transcription factors .
- Post-translational Modifications: Include phosphorylation and interactions with regulatory proteins like TPX2 .
Applications
Aurora kinases have significant applications in biomedical research, diagnostics, and therapeutics .
- Cancer Research: Due to their role in cell division, Aurora kinases are targets for cancer therapy .
- Diagnostic Tools: Elevated levels of Aurora kinases can serve as biomarkers for certain cancers .
- Therapeutic Strategies: Inhibitors of Aurora kinases are being developed and tested in clinical trials for cancer treatment .
