AURKA Human

Aurora Kinase A Human Recombinant
Cat. No.
BT27786
Source
Escherichia Coli.
Synonyms
Serine/threonine-protein kinase 6, Aurora kinase A, Serine/threonine kinase 15, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Aurora-A, Aurora-related kinase 1, hARK1, AURKA, AIK, ARK1, AURA, BTAK, STK15, STK6, STK7, STK15, AURORA2, MGC34538.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AURKA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 423 amino acids (1-403) and having a molecular mass of 47.9kDa. AURKA is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Aurora Kinase A (AURKA) is a crucial mitotic serine/threonine kinase that plays a vital role in cell division. As a member of the Aurora kinase family, AURKA is involved in various mitotic events, including microtubule formation and stabilization at spindle poles, ensuring proper chromosome segregation during cell division. Its expression is tightly regulated throughout the cell cycle, with low levels in the G1/S phase, accumulation during G2/M, and rapid decrease afterward. This precise regulation highlights its importance in cell cycle progression. AURKA is essential for both mitosis and meiosis, and its dysfunction can lead to severe consequences, including aneuploidy and tumorigenesis. AURKA is highly expressed in various cancer types, making it a potential target for cancer therapy.
Description
Recombinant AURKA, specifically the human variant, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 423 amino acids (residues 1-403) with a molecular weight of 47.9 kDa. For purification purposes, a 20 amino acid His-Tag is fused to the N-terminus of the AURKA protein. The protein is then purified using proprietary chromatographic techniques.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
AURKA is supplied in a solution containing 20mM Tris-HCl buffer with a pH of 8.0, 0.5mM DTT, 100mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF, and 20% glycerol.
Stability
AURKA is stable at 4°C for a period of 1 week. However, for long-term storage, it is recommended to store it at a temperature below -18°C. The addition of a carrier protein, such as HSA or BSA at a concentration of 0.1%, is advised for extended storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of AURKA is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Serine/threonine-protein kinase 6, Aurora kinase A, Serine/threonine kinase 15, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Aurora-A, Aurora-related kinase 1, hARK1, AURKA, AIK, ARK1, AURA, BTAK, STK15, STK6, STK7, STK15, AURORA2, MGC34538.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILALKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKRISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS.

Product Science Overview

Introduction

Aurora Kinase A (AURKA) is a serine/threonine kinase that plays a crucial role in cell division by regulating various processes during mitosis. It is a member of the Aurora kinase family, which also includes Aurora Kinase B and C. The recombinant form of Aurora Kinase A is often used in research to study its function and potential as a therapeutic target.

Structure and Expression

Aurora Kinase A is encoded by the AURKA gene located on chromosome 20q13.2. The protein is approximately 403 amino acids long and has a molecular weight of around 46 kDa. It is predominantly expressed in tissues with high mitotic activity, such as the testes and thymus, and its expression is tightly regulated during the cell cycle .

Function

Aurora Kinase A is essential for several key processes during mitosis:

  • Centrosome Maturation and Separation: It helps in the maturation of centrosomes and their separation, which is crucial for the formation of a bipolar spindle.
  • Spindle Assembly: It plays a role in the assembly and stabilization of the mitotic spindle, ensuring proper chromosome alignment and segregation.
  • Cytokinesis: Aurora Kinase A is involved in the final stages of cell division, aiding in the completion of cytokinesis .
Mechanism of Action

Aurora Kinase A exerts its effects by phosphorylating various substrates involved in mitosis. One of its well-known substrates is the microtubule-associated protein TPX2, which is essential for spindle assembly. The kinase activity of Aurora Kinase A is regulated by autophosphorylation and interaction with other proteins, such as Bora and Ajuba .

Clinical Significance

Aurora Kinase A has been implicated in cancer development and progression. Overexpression of AURKA is often observed in various cancers, including breast, colorectal, and pancreatic cancers. This overexpression is associated with poor prognosis and increased tumor aggressiveness. As a result, Aurora Kinase A is considered a potential therapeutic target, and several inhibitors are currently being developed and tested in clinical trials.

Recombinant Aurora Kinase A

Recombinant Aurora Kinase A is produced using various expression systems, such as baculovirus-insect cells or E. coli. The recombinant protein is used in biochemical assays to study its kinase activity, substrate specificity, and inhibition by potential therapeutic compounds. It is also used in structural studies to understand the molecular basis of its function and regulation.

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