AURKB Human
Aurora Kinase B Human Recombinant
Cat. No.
BT27871
Source
Escherichia Coli.
Synonyms
Serine/threonine-protein kinase 12, Aurora kinase B, Serine/threonine-protein kinase aurora-B, Aurora- and Ipl1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, AIM-1, ARK-2, STK-1, AURKB, AIK2, AIM1, ARK2, STK12, AurB, IPL1, STK5, aurkb-sv1, aurkb-sv2.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
AURKB Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 364 amino acids (1-344) and having a molecular mass of 41.4kDa. AURKB is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Aurora Kinase B (AURKB) is a mitotic serine/threonine kinase involved in chromosome segregation and cytokinesis. It regulates microtubule-kinetochore attachment and is crucial for the localization of BUB1 and SGOL1 to kinetochores. AURKB expression peaks during G2/M phase and is tightly regulated, with overexpression observed in various cancers. It phosphorylates histone H3 at Ser-10 and Ser-28 during mitosis and is part of the chromosomal passenger complex (CPC), a key regulator of mitosis. AURKB is highly expressed in the thymus, spleen, lung, testis, colon, placenta, and fetal liver. Its expression is upregulated in cancer cells during M phase.
Description
Recombinant AURKB protein (human origin) expressed in E. coli. This non-glycosylated polypeptide chain consists of 364 amino acids (residues 1-344), with a molecular weight of 41.4 kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The AURKB protein is supplied in a solution containing 20mM Tris-HCl buffer (pH 8.0), 0.5mM DTT, 20% glycerol, 0.1mM EDTA, 0.1mM EGTA, 0.1M NaCl, and 0.1mM PMSF.
Stability
AURKB is stable for 1 week when stored at 4°C. For long-term storage, it is recommended to store at temperatures below -18°C and add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Serine/threonine-protein kinase 12, Aurora kinase B, Serine/threonine-protein kinase aurora-B, Aurora- and Ipl1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, AIM-1, ARK-2, STK-1, AURKB, AIK2, AIM1, ARK2, STK12, AurB, IPL1, STK5, aurkb-sv1, aurkb-sv2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPMGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA.
Product Science Overview
Introduction
Aurora Kinase B (AURKB) is a member of the Aurora kinase family, which plays a crucial role in cell division by regulating chromosome segregation and cytokinesis. This serine/threonine kinase is essential for maintaining genomic stability and is a key player in mitosis.
Gene and Protein Structure
Expression and Localization
Biological Functions
Aurora Kinase B is involved in several critical processes during mitosis:
- Chromosome Condensation: Facilitates the condensation of chromosomes to ensure proper segregation.
- Spindle Assembly: Associates with microtubules near kinetochores, specifically the K-fibers, to aid in spindle assembly and stability .
- Cytokinesis: Plays a role in the final separation of daughter cells by regulating the contractile ring formation .
Mechanism of Action
Aurora Kinase B functions by phosphorylating various substrates involved in mitosis. One of its key targets is the mitotic centromere-associated kinesin (MCAK), which it phosphorylates on Ser92. This phosphorylation event inhibits MCAK’s microtubule depolymerizing activity, thereby stabilizing the spindle microtubules .
Clinical Significance
Overexpression of Aurora Kinase B has been linked to various cancers, as it can lead to aneuploidy and genomic instability. Consequently, AURKB is considered a potential target for anticancer therapies. Inhibitors of Aurora Kinase B are being explored as therapeutic agents to treat cancers characterized by high levels of this kinase .
Recombinant Aurora Kinase B
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