Enzymes
Product List
BPNT1 Human
3(2) 5-Bisphosphate Nucleotidase 1 Human Recombinant
BPNT1 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
NT5C2 Human
5'-Nucleotidase Cytosolic II Human Recombinant
NT5C2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 581 amino acids (1-561 a.a.) and having a molecular mass of 67.1kDa.
NT5C2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
NT5C3B Human
5'-Nucleotidase, Cytosolic IIIB Human Recombinant
NT5M Human
5',3'-Nucleotidase, Mitochondrial Human Recombinant
NT5M Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (32-228 a.a.) and having a molecular mass of 25.1kDa.
NT5M is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Key Biological Properties: Nucleotidases have broad substrate specificity and are multifunctional enzymes . They are involved in purine and pyrimidine salvage pathways, nucleic acid repair, cell-to-cell communication, and signal transduction .
Expression Patterns and Tissue Distribution: Nucleotidases are widely distributed across different cellular locations and are found in various tissues, including the plasma membrane, cytosol, and mitochondria . In mammalian cells, they are predominantly located in the plasma membrane .
Primary Biological Functions: Nucleotidases play a crucial role in the metabolism of nucleotides by converting extracellular nucleotides to nucleosides, which can readily enter cells . They are also involved in purine and pyrimidine salvage pathways, nucleic acid repair, and signal transduction .
Role in Immune Responses and Pathogen Recognition: Nucleotidases contribute to immune responses by regulating the levels of extracellular nucleotides, which can act as signaling molecules in immune cell activation and pathogen recognition .
Mechanisms with Other Molecules and Cells: Nucleotidases interact with various molecules and cells by hydrolyzing nucleotides to nucleosides and phosphate . They are involved in signal transduction cascades involving purinergic receptors .
Binding Partners and Downstream Signaling Cascades: Nucleotidases bind to substrates such as AMP, GMP, and IMP, and their activity is regulated by binding partners like ATP . They participate in downstream signaling cascades that regulate cellular metabolism and communication .
Regulatory Mechanisms: The expression and activity of nucleotidases are controlled by various regulatory mechanisms, including transcriptional regulation and post-translational modifications . ATP acts as an activator, increasing the enzyme’s affinity for AMP .
Transcriptional Regulation and Post-Translational Modifications: Nucleotidase activity is regulated at the transcriptional level by master transcription factors and at the enzyme level by allosteric regulation and feedback inhibition .
Biomedical Research: Nucleotidases are used in biomedical research to study nucleotide metabolism and signaling pathways .
Diagnostic Tools: They serve as biomarkers for various diseases, including cancer and metabolic disorders .
Therapeutic Strategies: Nucleotidases are targeted in therapeutic strategies to modulate nucleotide levels and treat conditions such as ischemia and inflammation .
Role Throughout the Life Cycle: Nucleotidases play a vital role throughout the life cycle, from development to aging and disease . They are involved in maintaining nucleotide balance, supporting DNA replication, and regulating cellular metabolism .
Development to Aging and Disease: During development, nucleotidases ensure proper nucleotide synthesis and repair. In aging, they help maintain cellular function and prevent nucleotide imbalances that can lead to diseases .
