NT5C2 Human
Cytosolic purine 5'-nucleotidase, Cytosolic 5'-nucleotidase II, NT5C2, NT5B, NT5CP, PNT5, GMP, cN-II.
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Description
NT5C2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 581 amino acids (1-561 a.a.) and having a molecular mass of 67.1kDa.
NT5C2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Cytosolic purine 5'-nucleotidase, Cytosolic 5'-nucleotidase II, NT5C2, NT5B, NT5CP, PNT5, GMP, cN-II.
MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E.
Product Science Overview
5’-Nucleotidase Cytosolic II, also known as NT5C2, is a crucial enzyme involved in the metabolism of nucleotides. This enzyme is responsible for the dephosphorylation of nucleoside monophosphates, primarily inosine monophosphate (IMP) and guanosine monophosphate (GMP), but also acts on adenosine monophosphate (AMP) to a lesser extent . The enzyme is encoded by the NT5C2 gene and is highly conserved across various species, indicating its fundamental role in cellular processes .
NT5C2 is a bifunctional enzyme that operates both as a phosphatase and a phosphotransferase . Its primary function is to maintain the balance of intracellular purine and pyrimidine compounds, which are essential for various cellular activities, including DNA and RNA synthesis . The enzyme’s activity is crucial for the regulation of nucleotide pools within the cell, ensuring that the correct balance of nucleotides is maintained for cellular functions .
The expression of NT5C2 is highly regulated and is particularly elevated in rapidly proliferating cells, such as tumor cells . This high expression level correlates with the enzyme’s role in supporting the increased nucleotide demand of proliferating cells . Additionally, NT5C2 has been implicated in chemotherapy resistance in certain cancers, particularly T-cell acute lymphoblastic leukemia, where mutations in the NT5C2 gene lead to overactive enzyme variants .
Human recombinant NT5C2 is typically produced using recombinant DNA technology. The gene encoding NT5C2 is cloned into an expression vector, which is then introduced into a suitable host cell, such as Escherichia coli or mammalian cells. The host cells are cultured under conditions that promote the expression of the recombinant protein, which is subsequently purified using various chromatographic techniques .
NT5C2 catalyzes the dephosphorylation of nucleoside monophosphates through a hydrolysis reaction, releasing inorganic phosphate and the corresponding nucleoside . The enzyme’s activity can be measured using various biochemical assays that detect the release of inorganic phosphate or the formation of nucleosides. These assays are essential for studying the enzyme’s kinetics and for screening potential inhibitors that could modulate its activity .
