BPNT1 Human

3(2) 5-Bisphosphate Nucleotidase 1 Human Recombinant
Cat. No.
BT15987
Source
Escherichia Coli.
Synonyms
3'(2'), 5'-bisphosphate nucleotidase 1, Bisphosphate 3'-nucleotidase 1, PAP-inositol-1,4-phosphatase, PIP, EC 3.1.3.7, BPntase.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BPNT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 344 amino acids (1-308a.a.) and having a molecular mass of 37.5kDa.
BPNT1 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
BPNT1, a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily, catalyzes the conversion of PAPS (adenosine 3'-phosphate 5' phosphosulfate) to APS (adenosine 5'-phosphosulfate) and PAP (3'(2')-phosphoadenosine 5' phosphate) to AMP (adenosine 5'-phosphate) using magnesium as a cofactor. Ubiquitously expressed, BPNT1 reaches its highest levels in the brain and kidney. The potent inhibition of BPNT1 by lithium, a drug used to treat manic depression and bipolar affective disorder, suggests a potential role for BPNT1 in the etiology of mood disorders.
Description
Recombinant BPNT1, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 344 amino acids (1-308a.a.) with a molecular mass of 37.5 kDa. The N-terminus of BPNT1 is fused to a 36 amino acid His-tag. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The BPNT1 protein solution (0.5 mg/mL) is formulated in 20 mM Tris-HCl buffer (pH 8.0) containing 5 mM DTT, 0.1 M NaCl, and 10% glycerol.
Purity
Greater than 90% as determined by SDS-PAGE.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. Avoid repeated freeze-thaw cycles.
Synonyms
3'(2'), 5'-bisphosphate nucleotidase 1, Bisphosphate 3'-nucleotidase 1, PAP-inositol-1,4-phosphatase, PIP, EC 3.1.3.7, BPntase.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS NTVLMRLVAS AYSIAQKAGM IVRRVIAEGD LGIVEKTCAT DLQTKADRLA QMSICSSLAR KFPKLTIIGE EDLPSEEVDQ ELIEDSQWEE ILKQPCPSQY SAIKEEDLVV WVDPLDGTKE YTEGLLDNVT VLIGIAYEGK AIAGVINQPY YNYEAGPDAV LGRTIWGVLG LGAFGFQLKE VPAGKHIITT TRSHSNKLVT DCVAAMNPDA VLRVGGAGNK IIQLIEGKAS AYVFASPGCK KWDTCAPEVI LHAVGGKLTD IHGNVLQYHK DVKHMNSAGV LATLRNYDYY ASRVPESIKN ALVP

Product Science Overview

Biological Properties

BPNT1 is primarily involved in the hydrolysis of 3’(2’)-phosphoadenosine 5’-phosphate (PAP) to adenosine monophosphate (AMP) and inositol 1,4-bisphosphate (Ins(1,4)P2) to inositol 4-phosphate . It also has the ability to hydrolyze adenosine 3’-phosphate 5’-phosphosulfate (PAPS) to adenosine 5’-phosphosulfate (APS) . This activity is crucial for preventing the toxic accumulation of PAP, which can inhibit various proteins, including PAPS-utilizing enzymes such as sulfotransferases and RNA processing enzymes .

Expression Patterns and Tissue Distribution

BPNT1 is expressed in various tissues, with significant expression in the testicular tissue, where it is also known as Testicular Tissue Protein Li 29 or Epididymis Luminal Protein 20 . The protein is highly conserved across species, with the human protein being 92% identical to its mouse counterpart .

Biological Functions

The primary function of BPNT1 is to regulate nucleotide metabolism through its phosphatase activity. By converting PAP to AMP, BPNT1 plays a role in inositol recycling and phosphoinositide metabolism . This regulation is essential for maintaining cellular homeostasis and preventing the accumulation of toxic intermediates .

Modes of Action

BPNT1’s enzymatic activity is magnesium-dependent, and it acts as a phosphatase to hydrolyze specific nucleotide substrates . Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPNT1, which may account for lithium-induced nephrotoxicity .

Regulatory Mechanisms

The activity of BPNT1 is regulated by inositol signaling pathways, which are crucial for its role in nucleotide metabolism . The inhibition of BPNT1 by lithium highlights the enzyme’s significance in therapeutic contexts and its potential impact on drug-induced side effects .

Associated Diseases

Mutations or dysregulation of BPNT1 have been associated with several diseases, including Brachydactyly, Type B1 and Fanconi Anemia, Complementation Group D1 . These associations underscore the importance of BPNT1 in maintaining normal cellular functions and its potential role in disease pathogenesis.

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