Enzymes
Product List
HDAC2 Human
Histone Deacetylase 2 Human Recombinant
The HDAC2 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
HDAC8 Human
Histone Deacetylase 8 Human Recombinant
HDAC8 Human Recombinant produced in Sf9 Baculovirus cells, glycosylated polypeptide chain containing 383 amino acids (1-377) and having a molecular mass of 42.6kDa. HDAC8 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
HDAC8 Mouse
Histone Deacetylase 8 Mouse Recombinant
HDAC8 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 383 amino acids (1-377 a.a.) and having a molecular mass of 42.5kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).
HDAC8 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction
Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from an ε-N-acetyl lysine amino acid on histone and non-histone proteins . This action is crucial for the regulation of gene expression, as it allows histones to wrap DNA more tightly, thereby repressing gene transcription . HDACs are classified into four main classes based on their sequence homology to yeast enzymes and domain organization :
- Class I: Includes HDAC1, HDAC2, HDAC3, and HDAC8. These are primarily located in the nucleus and are ubiquitously expressed.
- Class II: Subdivided into Class IIa (HDAC4, HDAC5, HDAC7, HDAC9) and Class IIb (HDAC6, HDAC10). These can shuttle between the nucleus and cytoplasm.
- Class III: Known as sirtuins (SIRT1-7), these are NAD±dependent deacetylases.
- Class IV: Contains only HDAC11, which shares properties with both Class I and II.
HDACs play a variety of roles in biological processes :
- Gene Expression: By removing acetyl groups from histones, HDACs cause chromatin condensation, leading to transcriptional repression .
- Immune Responses: HDACs are involved in the regulation of immune responses and pathogen recognition. For instance, HDAC9 plays a role in innate immunity .
- Cellular Processes: HDACs regulate cell proliferation, differentiation, migration, and apoptosis .
HDACs have significant applications in biomedical research and therapeutic strategies :
- Biomedical Research: HDAC inhibitors are used to study gene expression and chromatin dynamics.
- Diagnostic Tools: HDAC activity can serve as a biomarker for certain diseases.
- Therapeutic Strategies: HDAC inhibitors are being developed as treatments for cancer, neurodegenerative diseases, and inflammatory conditions .
HDACs play crucial roles throughout the life cycle, from development to aging and disease :
- Development: HDACs are essential for proper development and differentiation of various tissues.
- Aging: Changes in HDAC activity are associated with aging and age-related diseases.
- Disease: Dysregulation of HDAC activity is linked to several diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders .
