HDAC8 Human

Histone Deacetylase 8 Human Recombinant
Cat. No.
BT25076
Source

Sf9, Baculovirus cells.

Synonyms
Histone deacetylase 8, HD8, HDAC8, HDACL1, CDA07, RPD3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HDAC8 Human Recombinant produced in Sf9 Baculovirus cells, glycosylated polypeptide chain containing 383 amino acids (1-377) and having a molecular mass of 42.6kDa. HDAC8 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Histone deacetylase 8 (HDAC8) is a class 1 histone deacetylase that plays a crucial role in gene regulation. It participates in skull development and regulates the ERR-alpha/PGC1-alpha transcriptional complex, which is involved in metabolism. HDAC8 modifies histones, proteins that organize DNA and control gene accessibility. This modification process, known as acetylation/deacetylation, impacts DNA structure and the binding of transcription factors, ultimately influencing gene expression.
Description
Recombinant human HDAC8, produced in Sf9 insect cells using a baculovirus expression system, is a glycosylated protein with a molecular weight of 42.6 kDa. It encompasses amino acids 1-377 of the full-length protein and includes a 6-amino acid His-tag at the C-terminus for purification. The protein is purified to a high degree using proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The HDAC8 protein is provided at a concentration of 0.25 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the HDAC8 solution can be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To preserve protein stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It's important to avoid repeated freezing and thawing of the solution.
Purity
The purity of the HDAC8 protein is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
Histone deacetylase 8, HD8, HDAC8, HDACL1, CDA07, RPD3.
Source

Sf9, Baculovirus cells.

Amino Acid Sequence
MEEPEEPADS GQSLVPVYIY SPEYVSMCDS LAKIPKRASM VHSLIEAYAL HKQMRIVKPK VASMEEMATF HTDAYLQHLQ KVSQEGDDDH PDSIEYGLGY DCPATEGIFD YAAAIGGATI TAAQCLIDGM CKVAINWSGG WHHAKKDEAS GFCYLNDAVL GILRLRRKFE RILYVDLDLH HGDGVEDAFS FTSKVMTVSL HKFSPGFFPG TGDVSDVGLG KGRYYSVNVP IQDGIQDEKY YQICESVLKE VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY ILQWQLATLI LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP SCRPDRNEPH RIQQILNYIK GNLKHVVHHH HHH.

Product Science Overview

Introduction

Histone deacetylase 8 (HDAC8) is a member of the class I histone deacetylases (HDACs), which are crucial enzymes involved in the transcriptional regulation of gene expression in eukaryotic cells . HDACs catalyze the removal of acetyl groups from lysine residues on histones, leading to chromatin condensation and transcriptional repression .

Structure and Function

HDAC8 is a 43 kDa protein that is expressed in various tissues and has been shown to play a role in several biological processes . It functions on both histone and non-histone substrates, producing free lysine and acetate by catalyzing the hydrolysis of acetyllysine side chains . HDAC8 is involved in skull morphogenesis and the metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex .

Pathological Role

HDAC8 has been implicated in various diseases, particularly cancer. Overexpression and deregulation of HDAC8 contribute to cancer cell proliferation, metastasis, immune evasion, and drug resistance . Additionally, HDAC8 is associated with non-cancer diseases such as Cornelia de Lange Syndrome (CdLS), infectious diseases, cardiovascular diseases, pulmonary diseases, and myopathy .

Therapeutic Potential

Given its involvement in multiple diseases, HDAC8 is considered an attractive therapeutic target. Various HDAC8 selective inhibitors (HDAC8is) have been developed, showing promising anti-cancer effects . These inhibitors aim to restore normal acetylation levels, thereby reversing the pathological phenotypes associated with HDAC8 deregulation .

Recombinant HDAC8

Recombinant human HDAC8 is produced using baculovirus-infected insect cells and is available in carrier-free formulations . This recombinant protein is used in various research applications, including studying the enzyme’s activity and screening for potential inhibitors .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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