Introduction
Glucose-6-phosphate isomerase (GPI) is an enzyme that plays a crucial role in energy metabolism. It belongs to the GPI family, whose members are involved in energy pathways. GPI exists as a dimer and catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate. In mammals, GPI also acts as a cytokine secreted by tumors and an angiogenic factor (AMF) that promotes the movement of endothelial cells. Moreover, GPI functions as a neurotrophic factor called Neuroleukin, supporting the growth and survival of spinal and sensory neurons. GPI exhibits diverse roles both inside and outside the cell. Intracellularly, it participates in glycolysis and gluconeogenesis, while extracellularly, it acts as a neurotrophic factor for specific neuron types. Defects in the GPI gene can lead to nonspherocytic hemolytic anemia. Severe GPI deficiency is associated with hydrops fetalis, early neonatal death, and neurological impairment.
Description
This product consists of recombinant human GPI with a 20 amino acid His tag attached to its N-terminus. It is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 578 amino acids (residues 1-558). Its molecular weight is 65.3 kDa. The GPI protein undergoes purification using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that is sterile-filtered.
Formulation
The GPI solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product should be kept at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for extended storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 95%, as assessed by SDS-PAGE analysis.
Synonyms
Glucose-6-phosphate isomerase, Phosphoglucose isomerase, Phosphohexose isomerase, Autocrine motility factor, Neuroleukin, Sperm antigen 36, GPI, PGI, PHI, AMF, NLK, SA-36, GNPI.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ.
