CES1D Mouse

CES1D is expressed in various tissues, including the liver, colon, and brain . The recombinant form of this enzyme is often produced in HEK293 cells, which are human embryonic kidney cells commonly used for protein expression . The recombinant protein typically includes a His tag for purification purposes and has a calculated molecular weight of approximately 60.8 kDa, although the observed molecular weight can be around 53 kDa .

Carboxylesterases, including CES1D, have broad substrate specificities. They are involved in the metabolism of a wide variety of clinical drugs, illicit narcotics, and chemical nerve agents . CES1D specifically has been shown to hydrolyze p-nitrophenylacetate, with a specific activity greater than 11000 pmoles/min/μg .

In addition to their role in drug metabolism, carboxylesterases are also involved in lipid metabolism and energy homeostasis . Genetic manipulations and chemical inhibition studies in mice have demonstrated the importance of these enzymes in metabolic processes. For example, carboxylesterases have been implicated in the metabolism of lipid esters and the regulation of energy balance .

Recombinant CES1D is widely used in research to study its enzymatic properties and potential therapeutic applications. The enzyme’s ability to hydrolyze various substrates makes it a valuable tool for investigating drug metabolism and detoxification processes .

Recombinant CES1D is typically provided as a lyophilized powder, which should be stored at -20 to -80°C for long-term stability. Once reconstituted, the protein solution can be stored at 4-8°C for short-term use (2-7 days) or at -20°C for up to 3 months .