CES2E Mouse

Carboxylesterases, including CES2E, are characterized by their broad substrate specificity. They catalyze the hydrolysis of carboxylic esters into alcohol and carboxylic acid, a reaction that is crucial for the metabolism of various substances . The enzyme’s active site contains a serine residue, which plays a key role in the hydrolysis reaction.

CES2E, like other carboxylesterases, is involved in several physiological processes:

1. Detoxification: Carboxylesterases are essential for the detoxification of drugs and environmental toxicants. They hydrolyze ester-containing drugs into their active or inactive forms, facilitating their excretion from the body .
2. Lipid Metabolism: CES2E plays a significant role in lipid metabolism. It hydrolyzes lipid esters, contributing to lipid homeostasis and energy balance .
3. Metabolic Diseases: Recent research has highlighted the importance of carboxylesterases in metabolic diseases such as obesity and fatty liver disease. These enzymes are potential targets for the treatment of such disorders .

Recombinant expression of CES2E involves cloning the gene encoding the enzyme into an expression vector, which is then introduced into a host cell, typically E. coli or mammalian cells. The host cells produce the enzyme, which can be purified and used for various research applications .

Recombinant CES2E is used in various research studies to understand its role in metabolism and disease. It is also employed in drug development to study the metabolism of ester-containing drugs and to screen for potential inhibitors of the enzyme .