SUMF1 Human, Sf9
Sf9, Insect cells.
SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1.
Greater than 85.0% as determined by SDS-PAGE.
Description
SUMF1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 347 amino acids (34-374.a.) and having a molecular mass of 38.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). SUMF1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1.
Sf9, Insect cells.
SQEAGTGAGA GSLAGSCGCG TPQRPGAHGS SAAAHRYSRE ANAPGPVPGE RQLAHSKMVP IPAGVFTMGT DDPQIKQDGE APARRVTIDA FYMDAYEVSN TEFEKFVNST GYLTEAEKFG DSFVFEGMLS EQVKTNIQQA VAAAPWWLPV KGANWRHPEG PDSTILHRPD HPVLHVSWND AVAYCTWAGK RLPTEAEWEY SCRGGLHNRL FPWGNKLQPK GQHYANIWQG EFPVTNTGED GFQGTAPVDA FPPNGYGLYN IVGNAWEWTS DWWTVHHSVE ETLNPKGPPS GKDRVKKGGS YMCHRSYCYR YRCAARSQNT PDSSASNLGF RCAADRLPTM DHHHHHH.
Product Science Overview
The SUMF1 gene is located on chromosome 3p26.1 in humans . The gene encodes a protein that is part of the SUMF protein family. The primary function of SUMF1 is to catalyze the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, also known as C-alpha-formylglycine .
SUMF1 is essential for the activation of sulfatases, which are enzymes that catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates . The catalytic residue in the active site of eukaryotic sulfatases, C-alpha-formylglycine (FGly), is post-translationally generated from a cysteine by SUMF1 in the endoplasmic reticulum (ER) . This modification is critical for the proper functioning of sulfatases.
Mutations in the SUMF1 gene can lead to multiple sulfatase deficiency (MSD), a lysosomal storage disorder . This condition results from the inability to form the FGly residue, leading to inactive sulfatases and the accumulation of sulfated substrates in lysosomes . MSD is characterized by a range of symptoms, including developmental delays, skeletal abnormalities, and neurological deficits .
Recombinant SUMF1 produced in Sf9 cells (a cell line derived from the fall armyworm, Spodoptera frugiperda) is used for research and therapeutic purposes . The recombinant form retains the enzymatic activity necessary for the post-translational modification of sulfatases, making it a valuable tool for studying the function and regulation of these enzymes .
