POLR2I Human

POLR2I is a member of the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family . The human recombinant form of POLR2I is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 148 amino acids. It has a molecular mass of approximately 17.0 kDa . The recombinant protein is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .

POLR2I plays a critical role in the transcription process by contributing to the formation of the RNA polymerase II complex. This complex catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates . Specifically, POLR2I is involved in synthesizing mRNA precursors and various functional non-coding RNAs, which are essential for the regulation of gene expression .

The recombinant POLR2I protein is produced in E. coli and is purified using proprietary chromatographic techniques to ensure high purity and activity . The protein solution is typically formulated with 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol, and 1mM DTT to maintain stability . For long-term storage, it is recommended to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) and to avoid multiple freeze-thaw cycles .

POLR2I is widely used in laboratory research to study the mechanisms of transcription and gene regulation. It is also utilized in various biochemical assays to investigate the interactions between RNA polymerase II subunits and other transcription factors . The recombinant form of POLR2I allows researchers to conduct experiments with a consistent and reliable source of the protein, facilitating advancements in our understanding of transcriptional regulation.