POLR2F Human

POLR2F, also known as RPB6, is part of the clamp element of RNA polymerase II. It works in conjunction with parts of RPB1 and RPB2 to form a compartment that allows the attachment of the RPB4-RPB7 subcomplex . This structure stabilizes the transcribing polymerase on the DNA template, ensuring efficient and accurate transcription.

The human recombinant form of POLR2F is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 150 amino acids. It has a molecular mass of approximately 16.9 kDa, although its molecular size may appear higher on SDS-PAGE due to its structure .

The recombinant POLR2F is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification through proprietary chromatographic techniques. The resulting product is a sterile, filtered, colorless solution formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM dithiothreitol (DTT) .

For optimal stability, the POLR2F solution should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to freeze the solution at -20°C. To prevent degradation, it is advisable to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), and avoid multiple freeze-thaw cycles .

POLR2F is primarily used in laboratory research to study the mechanisms of transcription and the role of RNA polymerase II in gene expression. Its recombinant form allows researchers to investigate its function in a controlled environment, providing insights into its interactions with other subunits and transcription factors .