POLR2E Human

Polymerase II Polypeptide E Human Recombinant
Cat. No.
BT3009
Source
E.coli.
Synonyms
DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E, RPB5, RPABC1, hRPB25, hsRPB5.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POLR2E Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210) and having a molecular mass of 27.1kDa.
POLR2E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) is a member of the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. It acts as a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA, utilizing the four ribonucleoside triphosphates as substrates. POLR2E represents the fifth largest subunit of RNA polymerase II, the enzyme responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared among the three DNA-directed RNA polymerases and exists in a two-fold molar excess compared to other polymerase subunits. Notably, an interaction has been observed between the POLR2E subunit and a hepatitis virus transactivating protein, suggesting potential interactions between transcriptional activators and the polymerase through this subunit.
Description
Recombinant human POLR2E, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 233 amino acids (residues 1-210). It has a molecular weight of 27.1 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The POLR2E solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein, such as HSA or BSA (0.1%), is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the POLR2E protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E, RPB5, RPABC1, hRPB25, hsRPB5.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT
ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ.

Product Science Overview

Structure and Function

RNA polymerase II (RNAP II) is a multiprotein complex that transcribes DNA into precursors of mRNA, small nuclear RNA (snRNA), and microRNA . It is one of the three RNA polymerase enzymes found in the nucleus of eukaryotic cells. RNAP II is a 550 kDa complex composed of 12 subunits, making it the most studied type of RNA polymerase .

POLR2E, the fifth largest subunit of RNA polymerase II, plays a significant role in the transcription process. It interacts with various transcriptional activators and the polymerase itself, facilitating the transcription of DNA into RNA . This interaction is crucial for the proper functioning of the polymerase and the accurate synthesis of RNA.

Recombinant Production

The recombinant form of Polymerase II Polypeptide E is produced in Escherichia coli (E. coli) expression systems . The recombinant protein is typically a single, non-glycosylated polypeptide chain containing 233 amino acids and has a molecular mass of approximately 27.1 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification through chromatographic techniques .

Applications

Recombinant POLR2E is used in various research applications, including:

  • Blocking Experiments: It can be used as a control fragment in blocking experiments with corresponding antibodies .
  • Protein-Protein Interaction Studies: Its role in interacting with transcriptional activators makes it a valuable tool for studying protein-protein interactions in the transcription process .
  • Structural Studies: Understanding the structure and function of POLR2E can provide insights into the overall mechanism of RNA polymerase II and its role in gene expression .

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