POLR3F Human

POLR3F is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates . The human recombinant version of POLR3F is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 339 amino acids, with a molecular mass of approximately 38.1 kDa . It is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .

POLR3F is one of the many subunits that form the eukaryotic RNA polymerase III (RNA Pol III) complex . This complex is essential for the transcription of small RNA molecules, including 5S ribosomal RNA and tRNA . POLR3F binds to two subunits of the RNA polymerase III transcription initiation factor IIIB (TFIIIB), namely TFIIIB90 and TBP . This binding is crucial for the initiation of transcription by RNA polymerase III .

The recombinant POLR3F is formulated as a sterile, filtered, colorless solution containing 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 30% glycerol, and 1 mM DTT . It is recommended to store the solution at 4°C if it will be used within 2-4 weeks, or at -20°C for longer periods. For long-term storage, adding a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) is advised to avoid multiple freeze-thaw cycles .

POLR3F is primarily used in laboratory research to study the transcription mechanisms of RNA polymerase III and its role in gene expression . It is also utilized in various biochemical assays and structural studies to understand the interactions between different subunits of the RNA polymerase III complex .

POLR3F is intended for laboratory research use only and should not be used as drugs, agricultural or pesticidal products, food additives, or household chemicals . Proper safety measures should be taken while handling this recombinant protein to avoid any potential hazards .