POLR2D Human

Polymerase II Polypeptide D Human Recombinant
Cat. No.
BT2971
Source
E.coli.
Synonyms
HSRBP4, HSRPB4, RBP4, RPB16, DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, POLR2D.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POLR2D Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (1-142) and having a molecular mass of 18.7kDa.POLR2D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Polymerase II Polypeptide D (POLR2D) belongs to the eukaryotic RPB4 RNA polymerase subunit family. It represents the fourth largest subunit within RNA polymerase II. Pol II plays a crucial role as the primary component of the basal RNA polymerase II transcription machinery, responsible for synthesizing messenger RNA in eukaryotic cells. Studies in yeast indicate that the POLR2D subunit associates with the polymerase, particularly under suboptimal growth conditions, suggesting a potential role in stress protection. Functionally, POLR2D catalyzes the transcription of DNA into RNA utilizing the 4 ribonucleoside triphosphates as substrates.
Description
Recombinantly produced in E. coli, POLR2D Human is a single, non-glycosylated polypeptide chain. It comprises 165 amino acids (1-142) and exhibits a molecular mass of 18.7 kDa. The protein is engineered with a 23 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile-filtered solution, colorless in appearance.
Formulation
The POLR2D solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
Analysis by SDS-PAGE indicates a purity greater than 90%.
Synonyms
HSRBP4, HSRPB4, RBP4, RPB16, DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, POLR2D.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAGGSD PRAGDVEEDA SQLIFPKEFE TAETLLNSEV HMLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS LEGRFEDEEL QQILDDIQTK RSFQY.

Product Science Overview

Structure and Characteristics

The recombinant human POLR2D is typically produced in E. coli and is a single, non-glycosylated polypeptide chain. It contains 165 amino acids, with a molecular mass of approximately 18.7 kDa . The recombinant version often includes a His-tag at the N-terminus to facilitate purification through chromatographic techniques .

Function in Transcription

POLR2D is part of the RNA polymerase II complex, which is essential for the synthesis of messenger RNA (mRNA) in eukaryotic cells. This complex is composed of multiple subunits, and POLR2D plays a role in the assembly and stability of the polymerase complex. The RNA polymerase II complex is responsible for transcribing DNA into pre-mRNA, which undergoes further processing to become mature mRNA. This mRNA is then translated into proteins, which are essential for various cellular functions.

Applications in Research

Recombinant POLR2D is widely used in biochemical and molecular biology research. It is utilized to study the mechanisms of transcription and the role of RNA polymerase II in gene expression. Researchers use recombinant POLR2D to investigate the interactions between different subunits of the RNA polymerase II complex and to understand how mutations in these subunits can affect transcription and lead to various diseases.

Clinical Significance

Mutations or dysregulation of RNA polymerase II subunits, including POLR2D, can lead to various genetic disorders and diseases. Understanding the function and structure of POLR2D can provide insights into the molecular basis of these conditions and potentially lead to the development of targeted therapies.

In summary, Polymerase II Polypeptide D (Human Recombinant) is a vital tool in the study of transcription and gene expression. Its recombinant form allows researchers to explore the intricate details of RNA polymerase II function and its role in cellular processes.

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Source
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THE BIOTEK
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