POLR3K Human

Polymerase III Polypeptide K Human Recombinant
Cat. No.
BT3290
Source
Escherichia Coli.
Synonyms
DNA-directed RNA polymerase III subunit RPC10, RNA polymerase III subunit C10, DNA-directed RNA polymerase III subunit K, RNA polymerase III 12.5 kDa subunit, RPC12.5, RNA polymerase III subunit C11, HsC11p, RPC11, hRPC11, POLR3K, My010, Polymerase III Polypeptide K, C11, C11-RNP3, hRPC11, RPC10.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POLR3K Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 131 amino acids (1-108 a.a) and having a molecular mass of 14.7kDa.
POLR3K is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Polymerase III Polypeptide K, also known as PORL3K, is an essential subunit of RNA polymerase III. This subunit is small in size but critical for the enzyme's function. PORL3K's carboxy-terminal domain shares a high degree of similarity with the carboxy-terminal domain of an RNA polymerase II elongation factor. This structural similarity suggests a potential role in transcription elongation. Furthermore, PORL3K is involved in the cellular defense mechanism against intracellular bacterial and DNA viral infections. It acts as a sensor for both nuclear and cytosolic DNA, playing a crucial role in initiating the innate immune response.
Description
Recombinant human POLR3K, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 131 amino acids, with a sequence spanning from amino acid 1 to 108. The molecular weight of the recombinant protein is 14.7 kDa. For purification and detection purposes, a 23 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques, ensuring a high degree of purity.
Physical Appearance
The product is a clear, colorless solution that has been sterilized through filtration.
Formulation
The POLR3K protein is provided at a concentration of 0.25 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 50% glycerol, 2mM DTT, and 2mM EDTA.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein, such as HSA or BSA, at a concentration of 0.1% is advised for long-term storage. To maintain the integrity of the protein, avoid repeated freeze-thaw cycles.
Purity
The purity of the POLR3K protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
DNA-directed RNA polymerase III subunit RPC10, RNA polymerase III subunit C10, DNA-directed RNA polymerase III subunit K, RNA polymerase III 12.5 kDa subunit, RPC12.5, RNA polymerase III subunit C11, HsC11p, RPC11, hRPC11, POLR3K, My010, Polymerase III Polypeptide K, C11, C11-RNP3, hRPC11, RPC10.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D.

Product Science Overview

Structure and Function

POLR3K is composed of 108 amino acids and has a molecular weight of approximately 12.5 kDa . The carboxy-terminal domain of POLR3K shares a high degree of sequence similarity with the carboxy-terminal domain of an RNA polymerase II elongation factor . This similarity is not just structural but also functional, as POLR3K is required for proper pausing and termination during transcription .

Recombinant Production

The recombinant form of Polymerase III Polypeptide K is produced using Escherichia coli (E. coli) expression systems . The recombinant protein typically includes an N-terminal His-tag to facilitate purification and detection. The production process involves cloning the POLR3K gene into an expression vector, transforming the vector into E. coli cells, and inducing protein expression. The recombinant protein is then purified using affinity chromatography techniques.

Applications

Recombinant POLR3K is used extensively in research to study the mechanisms of transcription and the role of RNA polymerase III in gene expression. It is also used in structural biology to understand the interactions between different subunits of RNA polymerase III and other transcription factors.

Storage and Handling

Recombinant POLR3K is typically stored in a buffer containing Tris-HCl, NaCl, glycerol, DTT, and EDTA to maintain its stability and activity . It should be stored at 4°C for short-term use and at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to prevent protein degradation.

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