POLL Human

Polymerase (DNA directed), Lambda Human Recombinant
Cat. No.
BT2891
Source
E.coli.
Synonyms
Polymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.
POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DNA polymerase POLL plays a role in base excision repair (BER). This low-fidelity polymerase participates in both spontaneous and DNA damage-induced mutagenesis. Additionally, POLL is involved in the mutagenic bypass of T-T dimers. It exhibits terminal transferase and 5'-deoxyribose-5-phosphate lyase activity, which are coordinated by PCNA and replication protein A.
Description
Recombinant human POLL, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 320 amino acids (residues 1-300) with a molecular weight of 36.0 kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless, and filtered solution.
Formulation
The POLL solution is provided at a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 0.1 M NaCl, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Polymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW.

Product Science Overview

Structure and Production

The recombinant form of DNA Polymerase Lambda is typically produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain containing 320 amino acids, with a molecular mass of approximately 36.0 kDa . The recombinant enzyme is often fused to a 20 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .

Function

DNA Polymerase Lambda plays a crucial role in several DNA repair pathways, including:

  1. Base Excision Repair (BER): This pathway is responsible for repairing lesions that give rise to abasic (AP) sites in DNA . Pol λ has both template-dependent and template-independent (terminal transferase) DNA polymerase activities, which are essential for filling in the gaps left after the removal of damaged bases .

  2. Non-Homologous End Joining (NHEJ): Pol λ contributes to the repair of DNA double-strand breaks by NHEJ, a process that directly ligates the broken ends without the need for a homologous template .

  3. Homologous Recombination (HR): In addition to NHEJ, Pol λ is also involved in HR, a more accurate repair mechanism that uses a homologous sequence as a template for repair .

  4. 5’-Deoxyribose-5-Phosphate Lyase (dRP Lyase) Activity: Pol λ possesses dRP lyase activity, which is crucial for the removal of the 5’-deoxyribose phosphate group during BER .

Clinical Significance

Mutations or deficiencies in the POLL gene can lead to various genetic disorders. For instance, Split-Hand/Foot Malformation 1 and Adams-Oliver Syndrome have been associated with mutations in this gene . Additionally, the enzyme’s role in DNA repair pathways makes it a potential target for cancer therapy, as defects in these pathways are often linked to cancer development .

Research and Applications

Recombinant DNA Polymerase Lambda is widely used in research to study DNA repair mechanisms and to develop potential therapeutic interventions. Its ability to perform both template-dependent and template-independent DNA synthesis makes it a valuable tool in molecular biology and genetic engineering .

In summary, Polymerase (DNA directed), Lambda (Human Recombinant) is a versatile and essential enzyme involved in maintaining genomic stability through its roles in various DNA repair pathways. Its recombinant form provides a valuable resource for scientific research and potential therapeutic applications.

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