Enzymes
Product List
REV1 Human
REV1 Polymerase Human Recombinant
REV1 is fused to a 21 amino acid His-tag at N-terminus.
T7 RNAP
T7 RNA Polymerase Recombinant
T7 RNA polymerase Recombinant protein is produced by bacteriophage T7 DNA which is expressed in recombinant E. coli bacterial system
T7 RNA Polymerase is a DNA-dependent 5'→ 3' RNA polymerase which specifically recognizes T7 promoter sequences.
T7 Bacteriophage RNA Polymerase gene
TAF10 Human
TAF10 RNA Polymerase II Human Recombinant
TAF10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TAF9 Human
TAF9 Recombinant Human
TAF9 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Polymerases are enzymes that synthesize long chains of polymers or nucleic acids. They play a crucial role in the replication and transcription of genetic material. There are two main types of polymerases:
Key Biological Properties:
- DNA Polymerase: Essential for DNA replication, repair, and recombination. It adds nucleotides to the 3’ end of a DNA strand, ensuring accurate copying of genetic information .
- RNA Polymerase: Catalyzes the synthesis of RNA from a DNA template during transcription .
Expression Patterns and Tissue Distribution:
- DNA polymerases are ubiquitously expressed in all dividing cells, with higher expression in tissues with rapid cell turnover, such as the bone marrow and gastrointestinal tract .
- RNA polymerases are also widely expressed, with specific types (Pol I, Pol II, Pol III) having distinct roles in different tissues .
Mechanisms with Other Molecules and Cells:
- DNA polymerases interact with various proteins involved in the replication fork, such as helicases and primases .
- RNA polymerases interact with transcription factors and other regulatory proteins to initiate and regulate transcription .
Binding Partners and Downstream Signaling Cascades:
Biomedical Research:
- Polymerases are essential tools in molecular biology techniques such as PCR (Polymerase Chain Reaction) and DNA sequencing .
Diagnostic Tools:
- PCR-based diagnostic tests for infectious diseases and genetic disorders rely on the activity of DNA polymerases .
Therapeutic Strategies:
Throughout Development to Aging and Disease:
- DNA polymerases are crucial for cell division and development, ensuring accurate DNA replication .
- RNA polymerases are involved in the transcription of genes necessary for growth, development, and response to environmental changes .
- Dysregulation of polymerase activity can lead to diseases such as cancer and genetic disorders .
