TAF10 Human
TAF10 RNA Polymerase II Human Recombinant
Cat. No.
BT3466
Source
Escherichia Coli.
Synonyms
TAF10 RNA Polymerase II TATA Box Binding Protein (TBP)-Associated Factor 30kDa, Transcription Initiation Factor TFIID 30 KDa Subunit, STAF28, TAF2A, TAF2H.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TAF10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (84-218) and having a molecular mass of 16.9kDa.
TAF10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TAF10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
TAF10, a small subunit of TFIID, plays a crucial role in the formation of specific TFIID complexes. Research using human and mammalian cells has demonstrated that TAF10 is essential for transcriptional activation mediated by the estrogen receptor. Moreover, it is indispensable for cell cycle progression and various cellular differentiation processes.
Description
Recombinant human TAF10, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 158 amino acids (residues 84-218). It has a molecular weight of 16.9 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
The TAF10 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein such as 0.1% HSA or BSA is advised. It is crucial to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the product is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
TAF10 RNA Polymerase II TATA Box Binding Protein (TBP)-Associated Factor 30kDa, Transcription Initiation Factor TFIID 30 KDa Subunit, STAF28, TAF2A, TAF2H.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT
Product Science Overview
Function and Role in Transcription
The initiation of transcription by RNA polymerase II requires the coordinated activities of more than 70 polypeptides. TFIID, which includes TAF10, is essential for this process. TFIID binds to the core promoter region of genes to position RNA polymerase II correctly, serving as a scaffold for the assembly of the transcription complex and acting as a channel for regulatory signals .
TAF10, specifically, is involved in several critical functions:
- Basal Transcription: TAF10 participates in the basic transcriptional machinery, ensuring that the transcription process can begin.
- Coactivator Functions: It serves as a coactivator, helping to enhance the transcriptional activity of specific genes.
- Promoter Recognition: TAF10 aids in recognizing and binding to promoter regions of genes, facilitating the assembly of the transcription complex.
- Modification of General Transcription Factors (GTFs): It helps modify GTFs to facilitate the assembly and initiation of the transcription complex .
Interactions and Cellular Functions
TAF10 interacts with several other proteins and factors within the cell:
- TAF9: Another subunit of TFIID, which works closely with TAF10.
- SPT3 Homolog: A transcription initiation protein that interacts with TAF10.
- TAF13: Another TFIID subunit that interacts with TAF10.
- TATA Binding Protein (TBP): The core component of TFIID that binds to the TATA box in the promoter region .
Studies have shown that TAF10 is required for transcriptional activation by the estrogen receptor, progression through the cell cycle, and certain cellular differentiation programs .
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