Growth Factors
CD Antigens
Enzymes
Viral Antigens
Recombinant Proteins
Natural Proteins
Monoclonal Antibodies
Cytokines
Polyclonal Antibodies
Neurotrophins
Chemokines
Hormones
Enzymes
Product List
MGLL Human
Monoglyceride Lipase Human Recombinant
MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.
Shipped with Ice Packs 
Cat. No.
BT5869
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.
MGLL Human, Active
Monoglyceride Lipase Human Recombinant, Active
MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5946
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.
PLA1A Human
Phospholipase A1 Member A Human Recombinant
PLA1A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 454 amino acids (26-456) and having a molecular mass of 49.5kDa.
PLA1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PLA1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Introduction
Definition and Classification
Lipases are a group of enzymes that catalyze the hydrolysis of fats (lipids) into glycerol and free fatty acids. They are classified based on their source and substrate specificity:
- Pancreatic Lipase: Found in the pancreas, it plays a crucial role in the digestion of dietary fats.
- Hepatic Lipase: Located in the liver, it is involved in the metabolism of lipoproteins.
- Hormone-Sensitive Lipase: Present in adipose tissue, it is regulated by hormonal signals and is essential for mobilizing stored fats.
- Lipoprotein Lipase: Found in the endothelial cells of capillaries, it hydrolyzes triglycerides in lipoproteins.
Biological Properties
Key Biological Properties:
- Catalytic Activity: Lipases exhibit high specificity for the ester bonds in triglycerides.
- Stability: They are stable under a wide range of pH and temperature conditions.
Expression Patterns:
- Pancreatic Lipase: Expressed predominantly in the pancreas.
- Hepatic Lipase: Expressed in the liver.
- Hormone-Sensitive Lipase: Expressed in adipose tissue.
- Lipoprotein Lipase: Expressed in endothelial cells of capillaries.
Tissue Distribution:
- Pancreatic Lipase: Found in the pancreas and small intestine.
- Hepatic Lipase: Found in the liver and blood plasma.
- Hormone-Sensitive Lipase: Found in adipose tissue.
- Lipoprotein Lipase: Found in capillary endothelial cells.
Biological Functions
Primary Biological Functions:
- Fat Digestion: Pancreatic lipase breaks down dietary fats into absorbable units.
- Lipoprotein Metabolism: Hepatic and lipoprotein lipases are involved in the metabolism of lipoproteins, which transport fats in the blood.
- Fat Mobilization: Hormone-sensitive lipase mobilizes stored fats for energy production.
Role in Immune Responses:
- Pathogen Recognition: Lipases can recognize and hydrolyze lipid components of pathogen membranes, aiding in immune defense.
Modes of Action
Mechanisms with Other Molecules and Cells:
- Binding to Substrates: Lipases bind to triglycerides and hydrolyze the ester bonds.
- Interaction with Co-factors: Some lipases require co-factors such as bile salts for optimal activity.
Binding Partners:
- Colipase: Pancreatic lipase requires colipase for binding to lipid-water interfaces.
- Apolipoproteins: Lipoprotein lipase interacts with apolipoproteins on lipoprotein particles.
Downstream Signaling Cascades:
- Hormone-Sensitive Lipase: Activated by cyclic AMP (cAMP) signaling in response to hormonal signals like adrenaline.
Regulatory Mechanisms
Regulatory Mechanisms:
- Transcriptional Regulation: Gene expression of lipases is regulated by transcription factors such as PPARs (Peroxisome Proliferator-Activated Receptors).
- Post-Translational Modifications: Lipases can be activated or inhibited by phosphorylation, glycosylation, and other modifications.
Applications
Biomedical Research:
- Metabolic Studies: Lipases are used to study lipid metabolism and related disorders.
Diagnostic Tools:
- Enzyme Assays: Lipase activity assays are used to diagnose pancreatic diseases.
Therapeutic Strategies:
- Enzyme Replacement Therapy: Lipase supplements are used to treat conditions like exocrine pancreatic insufficiency.
Role in the Life Cycle
Role Throughout the Life Cycle:
- Development: Lipases are essential for the digestion and absorption of dietary fats, crucial for growth and development.
- Aging: Changes in lipase activity can affect lipid metabolism and contribute to age-related diseases.
- Disease: Dysregulation of lipase activity is associated with metabolic disorders such as obesity, diabetes, and cardiovascular diseases.
