Macrophage-Colony Stimulating Factor Human Recombinant
Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 37.1 KD. MCSF is purified by proprietary chromatographic techniques.
Macrophage Colony Stimulating Factor Human Recombinant, Baculovirus
Macrophage Colony Stimulating Factor Human Recombinant, His Tag
Macrophage Colony Stimulating Factor Human Recombinant, Sf9
MCSF produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 231 amino acids (33-255 a.a.) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
MCSF is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Insect cells.
Sterile filtered colorless solution.
Macrophage-Colony Stimulating Factor Mouse Recombinant
Macrophage-Colony Stimulating Factor Rat Recombinant
Macrophage Colony Stimulating Factor Rat Recombinant HEK
MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.
Macrophage Colony Stimulating Factor Mouse Recombinant, Sf9
MCSF Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 164 amino acids (33-187 aa) and having a molecular mass of 19.1 kDa.
MCSF is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Colony Stimulating Factor 1 Receptor Human Recombinant
MCSFR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 737 amino acids (20-517a.a.) and having a molecular mass of 82.1kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
MCSFR is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Sterile filtered colorless solution.
Cerebrospinal fluid (CSF) is a clear, colorless body fluid found within the tissue that surrounds the brain and spinal cord of all vertebrates . It is produced by specialized ependymal cells in the choroid plexus of the ventricles of the brain and absorbed in the arachnoid granulations . CSF acts as a shock absorber, providing mechanical and immunological protection to the brain inside the skull .
Key Biological Properties: CSF is an ultrafiltrate of plasma, containing water, proteins at low concentrations, ions, neurotransmitters, and glucose . It is produced continuously and circulates through the ventricular system of the brain and the subarachnoid space of the brain and spinal cord .
Expression Patterns and Tissue Distribution: CSF is primarily produced by the choroid plexus located in the lateral, third, and fourth ventricles of the brain . It circulates through the ventricles and subarachnoid space, providing a constant flow that is essential for its functions .
Primary Biological Functions: CSF cushions the brain and spinal cord, providing protection against mechanical shocks . It also plays a crucial role in removing metabolic waste, transporting neuromodulators and neurotransmitters, and maintaining homeostasis within the central nervous system (CNS) .
Role in Immune Responses and Pathogen Recognition: CSF provides basic immunological protection to the CNS by acting as a barrier against pathogens and facilitating the removal of waste products .
Mechanisms with Other Molecules and Cells: CSF interacts with various molecules and cells within the CNS. It is involved in the transport of neuromodulators and neurotransmitters, which are essential for neuronal communication .
Binding Partners and Downstream Signaling Cascades: The choroid plexus, which produces CSF, has specialized ependymal cells with apical villous projections that secrete the fluid . These cells are tightly bound to each other via tight junctions, ensuring the proper flow and function of CSF .
Regulatory Mechanisms Controlling Expression and Activity: The production and circulation of CSF are highly regulated by the choroid plexus and the arachnoid granulations . The motile cilia on the ependymal cells play a crucial role in propelling CSF through the ventricular system .
Transcriptional Regulation and Post-Translational Modifications: The regulation of CSF production involves various transcriptional and post-translational mechanisms that ensure the proper balance and flow of the fluid .
Biomedical Research: CSF is used in biomedical research to study the physiology and pathology of the CNS . It provides valuable insights into the immune responses and disease mechanisms within the brain and spinal cord .
Diagnostic Tools: CSF analysis is a critical diagnostic tool for various neurological disorders. It helps in diagnosing infections, autoimmune diseases, and malignancies by analyzing the composition and cell count of the fluid .
Therapeutic Strategies: CSF is used in therapeutic strategies to deliver drugs directly to the CNS, bypassing the blood-brain barrier . This approach is particularly useful in treating neurological diseases and brain tumors .
Role Throughout the Life Cycle: CSF plays a vital role throughout the life cycle, from development to aging and disease . During development, it provides essential nutrients and growth factors that support neural differentiation and proliferation . In aging, CSF helps maintain homeostasis and remove waste products, although its production and circulation may decline, contributing to age-related neurological disorders .