Growth Factors
Product List
Noggin Human
Noggin Human Recombinant
Noggin Human Recombinant produced in E.Coli is a non-glycosylated, non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains, having a total molecular mass of approximately 46.3kDa.
Noggin is purified by proprietary chromatographic techniques.
Noggin Human, HEK
Noggin Human Recombinant, HEK
Noggin produced in HEK293 cells is a polypeptide chain containing 211 amino acids (28-232a.a.) and having a molecular mass of 23.8kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
Noggin is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Noggin Human, Sf9
Noggin Human Recombinant, Sf9
Noggin produced in Sf9 Baculovirus cells is a glycosylated homodimer containing 205 amino acids and having a molecular mass of 47.9kDa under non-reducing conditions. (Molecular size on SDS-PAGE will appear at approximately 50-80kDa).
Noggin is purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Sterile Filtered White lyophilized (freeze-dried) powder.
Noggin Mouse
Noggin Mouse Recombinant
Introduction
Noggin, also known as NOG, is a protein encoded by the NOG gene in humans. It is a member of the cysteine knot superfamily and functions as an extracellular antagonist of bone morphogenetic proteins (BMPs). Noggin is highly conserved across species, including humans, mice, and Xenopus (an aquatic frog genus) .
Key Biological Properties: Noggin is a secreted protein that plays a crucial role in vertebrate dorsoventral patterning. It binds and inhibits the activity of BMPs, particularly BMP2, BMP4, BMP5, BMP6, BMP7, BMP13, and BMP14 .
Expression Patterns and Tissue Distribution: Noggin is expressed in various tissues, including nerve tissue, muscles, and bones. It is released from the notochord and regulates BMP4 during development . Increased plasma levels of Noggin have been observed in obese mice and in patients with a body mass index over 27 .
Primary Biological Functions: Noggin is essential for correct nervous system, somite, and skeletal development. It promotes somite patterning in the developing embryo and regulates the patterning of the neural tube and somites from the neural plate .
Role in Immune Responses and Pathogen Recognition: While Noggin’s primary functions are related to development, it also plays a role in learning, cognition, bone development, and neural tube fusion .
Mechanisms with Other Molecules and Cells: Noggin binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as BMP4. By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, Noggin creates morphogenic gradients .
Binding Partners and Downstream Signaling Cascades: Noggin binds to BMPs via a conserved N-terminal clip domain, preventing BMP binding to type I and type II serine-threonine kinase receptors and inhibiting signaling mediated by Smad1/5/8 .
Regulatory Mechanisms Controlling Expression and Activity: Noggin’s expression and activity are tightly regulated during development. It is released from the notochord and regulates BMP4 during development . Noggin can also bind to ActivinB, Nodal/Xnrs, and XWnt8, inactivating these pathways to induce head formation in Xenopus embryos .
Transcriptional Regulation and Post-Translational Modifications: Noggin’s transcription is regulated by various factors during development, ensuring its precise expression in specific tissues .
Biomedical Research: Noggin is used in research to study its role in development and disease. It has been shown to have anti-cancer effects by inhibiting BMP activity .
Diagnostic Tools and Therapeutic Strategies: Noggin is explored for its potential in bone regenerative therapies. It promotes osteogenesis in human adipose-derived mesenchymal stem cells via FGFR2/Src/Akt and ERK signaling pathways . Noggin recombinant proteins are used in functional assays, ELISA, immunohistochemistry, and Western blot .
Role Throughout the Life Cycle: Noggin is crucial during early embryonic development, particularly in neural induction and skeletal development. It continues to play a role in learning, cognition, and bone development throughout life . Noggin’s depletion in adipose tissue has been linked to obesity .
