Noggin Mouse

Noggin is a disulfide-linked homodimer, meaning it consists of two identical subunits connected by disulfide bonds . It functions primarily as an antagonist to Bone Morphogenetic Proteins (BMPs), particularly BMP-4, but also modulates the activities of other BMPs such as BMP-2, BMP-7, BMP-13, and BMP-14 . By binding to these BMPs, Noggin prevents them from interacting with their receptors, thereby inhibiting their signaling pathways .

Noggin’s ability to inhibit BMP signaling is critical during embryonic development. It helps regulate the formation of the neural tube, somites, and other structures by controlling the balance between BMP signaling and other pathways. This balance is essential for proper tissue differentiation and organogenesis .

Recombinant Mouse Noggin is produced using a mouse myeloma cell line (NS0) and is typically purified to a high degree of purity (>90%) using SDS-PAGE under reducing conditions . The recombinant protein is often used in research to study its effects on cell differentiation, tissue culture, and various bioassays.

Recombinant Mouse Noggin is widely used in scientific research due to its ability to inhibit BMP-induced processes. Some of its applications include:

• Cell Culture: Used to study the effects of BMP inhibition on various cell types.
• Bioassays: Employed in assays to measure its ability to inhibit BMP-induced alkaline phosphatase production in chondrogenic cells .
• Tissue Engineering: Investigated for its potential in promoting tissue regeneration and repair by modulating BMP signaling.

Recombinant Mouse Noggin is typically lyophilized and can be reconstituted in sterile PBS. It is stable for up to 12 months when stored at -20 to -70°C and for shorter periods at 2 to 8°C after reconstitution .