Growth Factors
Product List
SPP1 Human
Osteopontin Human Recombinant
The SPP1 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
SPP1 Human, Active
Osteopontin Human Recombinant, BioActive
SPP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (17-314 a.a.) and having a molecular mass of 36.2kDa.
SPP1 is fused to a 23 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
SPP1 Human, HEK
Osteopontin Human Recombinant, HEK
Osteopontin is purified by proprietary chromatographic techniques.
SPP1 Mouse
Osteopontin 1 Mouse Recombinant
SPP1 Mouse Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 287 amino acids (17-294 a.a) and having a molecular mass of 31.8kDa.
SPP1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction
Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a multifunctional glycoprotein encoded by the SPP1 gene. It belongs to the SIBLING (Small Integrin-Binding Ligand, N-linked Glycoprotein) family of proteins . OPN was initially identified in osteoblasts and is highly expressed in bone, but it is also found in various other tissues .
Key Biological Properties: OPN is a phosphorylated and glycosylated protein that undergoes extensive post-translational modifications . It has an arginine-glycine-aspartic acid (RGD) cell-binding sequence, a calcium-binding site, and two heparin-binding domains .
Expression Patterns: OPN is expressed by a variety of cell types, including osteoblasts, osteocytes, macrophages, endothelial cells, smooth muscle cells, and epithelial cells .
Tissue Distribution: OPN is found in almost every tissue in the body, including bone, skin, brain, body fluids, cartilage, and various tumors .
Primary Biological Functions: OPN plays a critical role in bone remodeling, biomineralization, and wound healing . It mediates cell adhesion, migration, and survival through interactions with integrins and CD44 receptors .
Role in Immune Responses: OPN functions as a Th1 cytokine, promoting cell-mediated immune responses and playing a role in chronic inflammatory and autoimmune diseases . It is involved in leukocyte recruitment and activation during immune responses .
Pathogen Recognition: OPN is associated with many inflammatory conditions caused by infections, allergic responses, autoimmunity, and tissue damage .
Mechanisms with Other Molecules and Cells: OPN interacts with multiple integrin receptors, including the vitronectin receptor (αvβ3) and various β1 and β5 integrins . It also binds to CD44 isoforms via multiple sites .
Binding Partners: OPN binds to integrins and CD44 receptors, initiating downstream signaling cascades . It can be cleaved by thrombin, exposing additional cryptic binding sites and producing functional chemotactic fragments .
Downstream Signaling Cascades: The binding of OPN to its receptors activates several downstream signaling pathways, including PI3K/AKT, FAK/AKT, and NF-κB, leading to cell proliferation, migration, and inflammation .
Transcriptional Regulation: The regulation of OPN expression involves various motifs in its promoter, including purine-rich sequences, ets-like sequences, glucocorticoid and vitamin D response elements, and interferon-inducible elements . Pro-inflammatory cytokines such as TNF-α and IL-1β strongly induce OPN expression .
Post-Translational Modifications: OPN undergoes extensive post-translational modifications, including phosphorylation, glycosylation, and cleavage . These modifications affect its function and interactions with other molecules .
Biomedical Research: OPN is widely studied in the context of bone remodeling, cancer, and immune responses . It serves as a biomarker for various diseases, including cancer and chronic inflammatory conditions .
Diagnostic Tools: OPN levels in plasma and serum are used as diagnostic markers for several diseases, including cancer and cardiovascular diseases .
Therapeutic Strategies: Targeting OPN and its signaling pathways is being explored as a therapeutic strategy for cancer, autoimmune diseases, and chronic inflammatory conditions .
Development: OPN is involved in implantation and placentation, playing a crucial role in the uterine-placental microenvironment during pregnancy .
Aging and Disease: OPN contributes to tissue maintenance, wound healing, and immune regulation throughout life . It is implicated in the pathogenesis of several conditions, including atherosclerosis, cancer, autoimmune disorders, chronic inflammation, and sepsis .
