SPP1 Mouse
HEK293 Cells.
Secreted Phosphoprotein-1, OPN, BNSP, BSPI, ETA-1, MGC110940, SPP-1, Osteopontin, Bone sialoprotein 1, Urinary stone protein, Nephropontin, Uropontin, SPP1
Greater than 90.0% as determined by SDS-PAGE.
Description
SPP1 Mouse Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 287 amino acids (17-294 a.a) and having a molecular mass of 31.8kDa.
SPP1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Osteopontin, also known as SPP1, is a glycoprotein with a significant role in bone remodeling. It is involved in immune responses in various cells like fibroblasts, macrophages, and lymphocytes, particularly during inflammation and wound healing. SPP1 exhibits strong binding affinity to hydroxyapatite, making it a crucial component of the mineralized matrix. It plays a vital role in cell-matrix interactions and protects against cardiac ischemia-reperfusion injury. Overexpression of SPP1 is observed in various cancers, including hepatocellular carcinoma and intrahepatic cholangiocarcinoma, and is associated with advanced tumor stages and poor prognosis. Elevated SPP1 levels are also linked to interstitial lung diseases.
Recombinant SPP1 Mouse, produced in HEK293 cells, is a single-chain glycosylated polypeptide. This protein comprises 287 amino acids (17-294 a.a), has a molecular weight of 31.8kDa, and includes a C-terminal 6-amino acid His tag. Purification is achieved through proprietary chromatographic methods.
The SPP1 solution is provided at a concentration of 0.25mg/ml in a buffer consisting of phosphate-buffered saline (pH 7.4) and 10% glycerol.
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For longer periods, it is recommended to store the solution at -20°C. To ensure stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is recommended. Repeated freezing and thawing should be avoided.
The purity of SPP1 is determined by SDS-PAGE analysis and is greater than 90%.
The biological activity of SPP1 is assessed by its ability to support HEK293 cell adhesion. This is determined by coating plates with mouse SPP1 and measuring the adhesion of HEK293 cells. The ED50 for this interaction is less than or equal to 1.5 µg/ml.
Secreted Phosphoprotein-1, OPN, BNSP, BSPI, ETA-1, MGC110940, SPP-1, Osteopontin, Bone sialoprotein 1, Urinary stone protein, Nephropontin, Uropontin, SPP1
HEK293 Cells.
DGSLPVKVTD SGSSEEKLYS LHPDPIATWL VPDPSQKQNL LAPQNAVSSE EKDDFKQETL PSNSNESHDH MDDDDDDDDD DGDHAESEDS VDSDESDESH HSDESDETVT ASTQADTFTP IVPTVDVPNG RGDSLAYGLR SKSRSFQVSD EQYPDATDED LTSHMKSGES KESLDVIPVA QLLSMPSDQD NNGKGSHESS QLDEPSLETH RLEHSKESQE SADQSDVIDS QASSKASLEH QSHKFHSHKD KLVLDPKSKE DDRYLKFRIS HELESSSSEV NHHHHHH
Product Science Overview
Osteopontin is a highly acidic, calcium-binding, phosphorylated glycoprotein. It contains an RGD (arginine-glycine-aspartate) tripeptide sequence at its NH2-terminal, which allows it to bind to RGD-dependent integrins . The protein is expressed in various tissues, including bone, kidney, and immune cells. In mice, the recombinant form of Osteopontin is produced using mouse myeloma cell lines or 293E cells .
Osteopontin has several critical functions:
- Bone Remodeling: It is involved in the regulation of bone mineralization and resorption. Osteopontin binds tightly to hydroxyapatite, forming an integral part of the mineralized matrix .
- Immune Response: OPN plays a role in recruiting and stimulating macrophages and lymphocytes in response to microbial infections. It modulates cytokine production and promotes type I immunity .
- Cell Migration and Survival: The protein interacts with integrins and CD44 as receptors, facilitating cell migration and survival. It is involved in processes such as wound healing and tissue repair .
- Cancer Progression: Elevated levels of Osteopontin have been associated with the progression of various cancers. It is involved in the control of tumor cell phenotypes and metastasis .
- Atherosclerosis: OPN is a component of human atherosclerotic plaques and may mediate arterial neointima formation. It is synthesized by macrophages, smooth muscle, and endothelial cells in atherosclerotic plaques .
Recombinant Mouse Osteopontin is produced using advanced biotechnological methods. The protein is expressed in cell lines such as NS0 or 293E cells and purified to high levels of purity (>95%) using techniques like SDS-PAGE . The recombinant protein is available in both carrier-free and carrier-containing formulations, with Bovine Serum Albumin (BSA) often used as a carrier protein to enhance stability and shelf-life .
