SPP1 Human

Osteopontin Human Recombinant
Cat. No.
BT25506
Source
Escherichia Coli.
Synonyms
Secreted Phosphoprotein-1, OPN, BNSP, BSPI, ETA-1, MGC110940, SPP-1, Osteopontin, Bone sialoprotein 1, Urinary stone protein, Nephropontin, Uropontin, SPP1.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Secreted Phosphoprotein-1 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 321 amino acids fragment (17-314) and having a total molecular mass of 36.2 kDa(molecular weight on SDS-PAGE will shift up).
The SPP1 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Osteopontin, also known as Secreted Phosphoprotein-1 (SPP1), is a glycoprotein with a significant role in bone remodeling. Initially discovered in osteoblasts, it actively participates in the immune response of fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. SPP1 exhibits strong binding affinity to hydroxyapatite, making it a crucial component of the mineralized matrix and cell-matrix interactions. Furthermore, SPP1 plays a protective role against cardiac ischemia-reperfusion injury through late preconditioning. Overexpression of SPP1 is associated with advanced tumor stages in hepatocellular carcinoma, and it serves as a prognostic indicator. It is notably the most highly expressed gene in intrahepatic cholangiocarcinoma. Elevated levels of SPP1 are also linked to interstitial lung diseases.
Description
This product consists of a single, non-glycosylated polypeptide chain of human Secreted Phosphoprotein-1 (SPP1), recombinantly produced in E. coli. The protein encompasses amino acids 17-314, representing a fragment of the full SPP1 sequence. It has a molecular weight of 36.2 kDa, although this may appear higher on SDS-PAGE due to the presence of a 20 amino acid His-Tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
Osteopontin is provided in a buffer solution containing 20mM Tris-HCl (pH 7.5), 1mM DTT, 2mM EDTA, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. It's important to avoid repeated freezing and thawing of the product.
Purity
The purity of this product is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Secreted Phosphoprotein-1, OPN, BNSP, BSPI, ETA-1, MGC110940, SPP-1, Osteopontin, Bone sialoprotein 1, Urinary stone protein, Nephropontin, Uropontin, SPP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N.

Product Science Overview

Structure and Function

Osteopontin contains an arginine-glycine-aspartic acid (RGD) sequence, which is essential for its interaction with integrins. This interaction facilitates cell adhesion and migration, making osteopontin a key player in wound healing and tissue repair . Additionally, osteopontin is involved in bone mineralization by binding to hydroxyapatite, a major component of bone .

Recombinant Osteopontin

Recombinant human osteopontin is produced using various expression systems, including HEK293 cells and mouse myeloma cell lines. The recombinant protein is typically purified to high levels of purity, often exceeding 95%, and is used in various research applications . The recombinant form of osteopontin retains its functional properties, including its ability to enhance cell adhesion and support cell migration .

Applications in Research

Recombinant osteopontin is widely used in research to study its role in various biological processes. It is utilized in cell adhesion assays, migration studies, and investigations into its involvement in chronic inflammatory diseases and cancer . The protein’s ability to interact with integrins and CD44 makes it a valuable tool for understanding cell signaling pathways and developing therapeutic strategies.

Clinical Implications

Osteopontin has been implicated in several diseases, including cancer, cardiovascular diseases, and autoimmune disorders. Its overexpression is often associated with tumor progression and metastasis, making it a potential biomarker for cancer diagnosis and prognosis . Additionally, osteopontin’s role in inflammation and immune responses highlights its potential as a therapeutic target for inflammatory diseases .

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