SPP1 Human, Active
OPN, SPP-1, BNSP, BSPI, ETA-1, Bone sialoprotein 1, BSP I.Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Secreted phosphoprotein 1, SPP 1, SPP1, urinary stone protein, uropontin.
Description
SPP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (17-314 a.a.) and having a molecular mass of 36.2kDa.
SPP1 is fused to a 23 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Osteopontin, also known as SPP1, is a glycoprotein with a key role in bone remodeling. Primarily found in osteoblasts, it is also present in fibroblasts, macrophages, and lymphocytes, contributing to immune responses during inflammation and wound healing. SPP1 exhibits strong binding affinity to hydroxyapatite, an essential component of the mineralized matrix, and is crucial for cell-matrix interactions. Furthermore, SPP1 provides protection against cardiac ischemia-reperfusion injury through late preconditioning. Elevated levels of SPP1 and CD44 in hepatocellular carcinoma are associated with advanced disease stage and offer prognostic insights. Notably, SPP1 is significantly overexpressed in intrahepatic cholangiocarcinoma and has been linked to interstitial lung diseases.
Recombinant Human SPP1, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing 321 amino acids (residues 17-314). With a molecular weight of 36.2 kDa, this protein includes a 23 amino acid His tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
The SPP1 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 7.5), 1mM DTT, 10% glycerol, and 2mM EDTA.
The biological activity of this product is evaluated by its ability to support the adhesion of HEK293 human embryonic kidney cells. When HEK293 cells are added to plates coated with 10ug/ml of OPN, a significant increase in cell adhesion, exceeding 40%, is observed.
OPN, SPP-1, BNSP, BSPI, ETA-1, Bone sialoprotein 1, BSP I.Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Secreted phosphoprotein 1, SPP 1, SPP1, urinary stone protein, uropontin.
MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N.
Product Science Overview
Osteopontin is a highly phosphorylated glycoprotein that can bind to integrins and CD44 receptors on the cell surface. The human recombinant form of OPN is produced using a mouse myeloma cell line (NS0) and is typically tagged with a C-terminal 6-His tag for purification purposes . The recombinant protein is often used in research to study its biological functions and interactions.
OPN is involved in a wide range of biological functions, including:
- Bone Remodeling: OPN mediates the adhesion of osteoblasts and osteoclasts, playing a critical role in bone synthesis and resorption .
- Immune Response: OPN is expressed by various immune cells, including macrophages, T cells, and dendritic cells. It is involved in both innate and adaptive immune responses, contributing to inflammation and tissue repair .
- Wound Healing: OPN promotes cell migration and survival, making it essential for wound healing and tissue regeneration .
- Cancer: OPN is overexpressed in several types of cancer and is associated with tumor progression and metastasis .
The expression of OPN is regulated by various cytokines, growth factors, and transcription factors. For example, transforming growth factor-beta (TGF-β) and interleukin-1 (IL-1) are known to upregulate OPN expression. Additionally, OPN expression is modulated by mechanical stress and extracellular matrix components .
Due to its involvement in numerous physiological and pathological processes, OPN is a potential biomarker and therapeutic target for various diseases, including:
- Rheumatoid Arthritis: OPN is implicated in the pathogenesis of rheumatoid arthritis and is considered a potential therapeutic target .
- Osteoarthritis: Elevated levels of OPN are associated with the progression of osteoarthritis .
- Cancer: OPN is a potential biomarker for cancer diagnosis and prognosis, as well as a target for cancer therapy .
In conclusion, Osteopontin (Human Recombinant, BioActive) is a versatile protein with significant roles in bone remodeling, immune responses, and tissue repair. Its wide distribution and regulatory mechanisms make it a critical molecule in both health and disease.
