Growth Factors
Product List
NOV Human
Nephroblastoma Overexpressed Human Recombinant
NOV Human, HEK
Nephroblastoma Overexpressed Human Recombinant, HEK
NOV Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 33-357) containing 331 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 36.5kDa (calculated).
HEK293 cells.
NOV Mouse
Nephroblastoma Overexpressed Mouse Recombinant
The NOV is purified by proprietary chromatographic techniques.
Introduction
NOV, also known as CCN3 (Cellular Communication Network Factor 3), is a member of the CCN family of secreted, extracellular matrix (ECM)-associated signaling proteins. The CCN family includes six members: CYR61 (CCN1), CTGF (CCN2), NOV (CCN3), WISP1 (CCN4), WISP2 (CCN5), and WISP3 (CCN6) . These proteins are characterized by their multimodular structure, which includes domains homologous to insulin-like growth factor binding proteins (IGFBPs), von Willebrand factor type C repeats (vWC), thrombospondin type 1 repeats (TSR), and a cysteine knot motif within the C-terminal (CT) domain .
NOV is a matricellular protein that plays a crucial role in various cellular activities. It is expressed in multiple tissues, including the kidney, brain, and bone marrow . The expression patterns of NOV are tightly regulated during development and in response to physiological stimuli. NOV is involved in cell adhesion, migration, proliferation, differentiation, and survival . It is also expressed during wound healing and induces angiogenesis in vivo .
NOV regulates multiple cellular activities through direct binding to integrin receptors and other receptors such as NOTCH1 and fibulin 1c (FBLN1) . It is essential for the self-renewal of CD34+ hematopoietic stem cells from umbilical cord blood . NOV can bind BMP2 and inhibit its functions in promoting osteogenic differentiation, and stimulate osteoclastogenesis through a process that may involve calcium flux . Additionally, NOV is involved in regulatory T cell-mediated oligodendrocyte differentiation in the regeneration of myelin following damage to the myelin sheath .
NOV functions by interacting with various molecules and cells. It binds to integrin receptors, NOTCH1, and fibulin 1c (FBLN1), initiating downstream signaling cascades that regulate cellular activities . NOV can also bind BMP2, inhibiting its osteogenic functions and stimulating osteoclastogenesis . These interactions highlight the diverse roles of NOV in cellular signaling and regulation.
The expression and activity of NOV are regulated by multiple mechanisms. Transcriptional regulation involves the hematopoietic transcription factor MZF-1 . Post-translational modifications, such as phosphorylation, also play a role in modulating NOV’s activity . These regulatory mechanisms ensure that NOV’s expression and function are tightly controlled in response to physiological and pathological stimuli.
NOV has significant applications in biomedical research, diagnostic tools, and therapeutic strategies. Its role in cell adhesion, migration, and differentiation makes it a valuable target for cancer research and therapy . NOV’s involvement in wound healing and angiogenesis also presents opportunities for developing novel treatments for tissue repair and regeneration . Additionally, NOV’s regulatory functions in immune responses and stem cell renewal highlight its potential in developing therapies for immune-related disorders and regenerative medicine .
Throughout the life cycle, NOV plays critical roles from development to aging and disease. During development, NOV is involved in tissue differentiation and organogenesis . In adulthood, NOV contributes to tissue homeostasis and repair, particularly in response to injury . In aging and disease, dysregulation of NOV expression and function can contribute to pathological conditions such as cancer and degenerative diseases . Understanding NOV’s role in these processes provides insights into its potential as a therapeutic target.
