NOV Human
Nephroblastoma Overexpressed Human Recombinant
Cat. No.
BT24557
Source
Escherichia Coli.
Synonyms
Protein NOV homolog, NovH, CCN family member 3, nsulin-like growth factor-binding protein 9, IBP-9, IGF-binding protein 9, IGFBP-9, Nephroblastoma-overexpressed gene protein homolog, NOV, CCN3, IGFBP9, NOVH.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Nephroblastoma Overexpressed Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 331 amino acids and having a molecular mass of 36.2 kDa. The NOV is purified by proprietary chromatographic techniques.
Product Specs
Introduction
The NOV gene encodes Nephroblastoma Overexpressed (NOV), a member of the CCN (CTGF/CYR61/NOV) family. NOV plays a role in suppressing tumor growth and proliferation in certain cancer cell lines. It interacts with various proteins and participates in both intracellular and extracellular signaling pathways. NOV expression is observed in specific tumors, including Wilms' tumor and a majority of nephroblastomas. Additionally, it exhibits proangiogenic properties.
Description
Recombinant Human Nephroblastoma Overexpressed, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 331 amino acids. It has a molecular weight of 36.2 kDa. The purification of NOV is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2µm filtered solution in 20mM Tris-HCl, pH 8.6 and 150 mM NaCl.
Solubility
Reconstitute the lyophilized NOV in sterile 18M-cm H₂O at a concentration of at least 100µg/ml. Further dilutions can be made in other aqueous solutions.
Stability
Lyophilized NOV is stable at room temperature for 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, store NOV at 4°C for 2-7 days. For long-term storage, store below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The biological activity, assessed using a cell proliferation assay with murine Balb/c 3T3 cells, is less than 1.0 µg/ml. This corresponds to a specific activity greater than 1000 IU/mg. A typical ED50 is in the range of 10.0 - 50.0 ng/ml, which translates to a specific activity of 20,000 - 100,000 units/mg.
Synonyms
Protein NOV homolog, NovH, CCN family member 3, nsulin-like growth factor-binding protein 9, IBP-9, IGF-binding protein 9, IGFBP-9, Nephroblastoma-overexpressed gene protein homolog, NOV, CCN3, IGFBP9, NOVH.
Source
Escherichia Coli.
Amino Acid Sequence
MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M.
Product Science Overview
Expression and Tissue Distribution
NOV is expressed in several tissues and cell types, including the kidney, brain, and bone marrow. It is particularly notable for its expression in certain tumors, such as Wilms’ tumor and nephroblastomas . The protein is involved in both internal and external cell signaling, interacting with various proteins to mediate its effects.
Biological Functions
NOV has been implicated in several biological functions:
- Tumor Suppression: NOV is known to reduce the tumorigenicity and proliferation of certain cancer cell lines .
- Angiogenesis: It has proangiogenic activities, promoting the formation of new blood vessels .
- Cell Proliferation: NOV influences cell proliferation, as evidenced by its activity in cell proliferation assays using murine Balb/c 3T3 cells .
Recombinant NOV
Recombinant human NOV (rHuNOV) is produced using Escherichia coli as the expression system. The recombinant protein is a single non-glycosylated polypeptide chain containing 331 amino acids, with a molecular weight of approximately 36.2 kDa . It is highly purified, with a purity of over 95% as determined by SDS-PAGE and HPLC analyses .
Applications
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