NOV Human, HEK
HEK293 cells.
Protein NOV homolog, NovH, CCN family member 3, nsulin-like growth factor-binding protein 9, IBP-9, IGF-binding protein 9, IGFBP-9, Nephroblastoma-overexpressed gene protein homolog, NOV, CCN3, IGFBP9, NOVH.
Greater than 90.0% as determined by SDS-PAGE.
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Description
NOV Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 33-357) containing 331 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 36.5kDa (calculated).
Product Specs
Nephroblastoma Overexpressed (NOV), encoded by the NOV gene, belongs to the CCN (CTGF/CYR61/NOV) family. NOV plays a role in suppressing tumor development and the rapid multiplication of cells in certain cancer cell lines. It interacts with various proteins and participates in both intracellular and extracellular signaling pathways. NOV is found in specific tumors, including Wilms' tumor and most nephroblastomas. Additionally, it exhibits proangiogenic properties, promoting the formation of new blood vessels.
This product consists of human recombinant NOV protein produced in HEK293 cells. It is a single, glycosylated polypeptide chain with a molecular weight of 36.5kDa (calculated). The protein sequence spans amino acids 33 to 357 and includes a 6-amino acid C-terminal His tag.
The NOV protein has undergone filtration (0.4 µm) and lyophilization. The initial concentration before lyophilization was 0.5mg/ml in a solution of PBS with 5% (w/v) trehalose.
To prepare a working stock solution, it is advised to add deionized water to the lyophilized pellet until it fully dissolves, achieving an approximate concentration of 0.5mg/ml. Please note that this product is not sterile. Before using it in cell culture, it is essential to filter the solution through an appropriate sterile filter.
For long-term storage, the lyophilized protein should be kept at -20°C. After reconstituting the protein, it is recommended to aliquot it into smaller portions to minimize repeated freezing and thawing cycles. The reconstituted protein can be stored at 4°C for a limited duration, maintaining stability for up to one week.
Analysis by SDS-PAGE indicates that the purity of this product is greater than 90.0%.
Protein NOV homolog, NovH, CCN family member 3, nsulin-like growth factor-binding protein 9, IBP-9, IGF-binding protein 9, IGFBP-9, Nephroblastoma-overexpressed gene protein homolog, NOV, CCN3, IGFBP9, NOVH.
HEK293 cells.
QRCPPQCPGR CPATPPTCAP GVRAVLDGCS CCLVCARQRG ESCSDLEPCD ESSGLYCDRS ADPSNQTGIC TAVEGDNCVF DGVIYRSGEK FQPSCKFQCT CRDGQIGCVP RCQLDVLLPE PNCPAPRKVE VPGECCEKWI CGPDEEDSLG GLTLAAYRPE ATLGVEVSDS SVNCIEQTTE WTACSKSCGM GFSTRVTNRN RQCEMLKQTR LCMVRPCEQE PEQPTDKKGK KCLRTKKSLK AIHLQFKNCT SLHTYKPRFC GVCSDGRCCT PHNTKTIQAE FQCSPGQIVK KPVMVIGTCT CHTNCPKNNE AFLQELELKT TRGKMHHHHH H.
Product Science Overview
Nephroblastoma Overexpressed (NOV), also known as CCN3, is a member of the CCN family of proteins. This family includes connective tissue growth factor (CTGF/CCN2), cysteine-rich angiogenic inducer 61 (CYR61/CCN1), and nephroblastoma overexpressed (NOV/CCN3). These proteins are involved in various cellular processes, including cell adhesion, migration, proliferation, and differentiation .
The NOV gene encodes a matricellular protein that is involved in the regulation of cell-matrix interactions. The protein structure of NOV includes several conserved domains: an insulin-like growth factor-binding protein (IGFBP) domain, a von Willebrand factor type C (vWC) domain, a thrombospondin type 1 (TSP1) domain, and a cysteine knot (CT) domain. These domains are crucial for the protein’s interaction with other cellular components and its role in cellular signaling pathways .
NOV/CCN3 has been shown to have growth-inhibiting properties. It plays a role in regulating extracellular matrix protein expression and influences its own expression when overexpressed. The protein is involved in various biological processes, including tissue repair, fibrosis, and tumorigenesis. NOV/CCN3 acts as a downstream mediator of transforming growth factor-beta 1 (TGF-β1) signaling, which is involved in tissue scarring and the stimulation of extracellular matrix protein deposition .
Human embryonic kidney (HEK) cells are commonly used for the recombinant expression of proteins due to their high transfection efficiency and ability to perform post-translational modifications. In the case of NOV/CCN3, stable HEK cell lines have been established to produce recombinant human NOV/CCN3 protein. These cell lines are used to generate large quantities of the protein for research and therapeutic purposes .
The recombinant NOV/CCN3 protein is purified using various chromatographic techniques. The identity of the purified protein is confirmed through in-gel digestion followed by mass spectrometry. Functional characterization of the protein is performed using assays such as the Smad3-sensitive reporter gene assay and the BrdU proliferation assay. These assays help determine the biological activity of the recombinant protein .
The recombinant NOV/CCN3 protein has potential applications in the diagnosis and treatment of fibrotic diseases and cancer. Its role in regulating extracellular matrix protein expression makes it a promising target for therapeutic interventions. Further research is needed to fully understand the mechanisms by which NOV/CCN3 influences cellular processes and to develop effective therapies based on this protein .
