NOV Mouse
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Description
The NOV is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Nephroblastoma Overexpressed (NOV), also known as CCN3, is a matricellular protein encoded by the NOV gene. This protein plays a crucial role in various cellular activities, including cell adhesion, proliferation, differentiation, migration, and survival. The recombinant form of this protein, derived from mice, is used extensively in research to understand its functions and potential therapeutic applications.
The NOV protein consists of 357 amino acids and includes an N-terminal secretory signal peptide. It has four distinct domains:
- Insulin-like Growth Factor Binding Protein (IGFBP) domain
- Thrombospondin Type 1 Repeat (TSR) domain
- von Willebrand Type C (vWC) repeats
- Cysteine Knot Motif within the C-terminal (CT) domain
These domains enable NOV to interact with various receptors, such as integrin receptors, NOTCH1, and fibulin 1c (FBLN1). NOV is involved in wound healing, angiogenesis, and the self-renewal of CD34+ hematopoietic stem cells from umbilical cord blood .
NOV regulates multiple cellular activities:
- Cell Adhesion and Migration: NOV binds to integrin receptors, facilitating cell adhesion and migration.
- Proliferation and Differentiation: It influences cell proliferation and differentiation by interacting with various signaling pathways.
- Angiogenesis: NOV induces angiogenesis, promoting the formation of new blood vessels.
- Osteogenesis and Osteoclastogenesis: NOV can bind BMP2, inhibiting its function in promoting osteogenic differentiation. It also stimulates osteoclastogenesis, potentially involving calcium flux .
NOV-null mice are viable and largely normal, exhibiting only modest and transient sexually dimorphic skeletal abnormalities. However, they show enhanced blood vessel neointimal thickening when challenged with vascular injury, indicating that NOV inhibits neointimal hyperplasia .
In cancer, NOV has a dual role. While it inhibits the proliferation of cancer cells, it also promotes metastasis. Overexpression of NOV results in reduced tumor size in glioma cell xenografts but enhances metastatic potential in melanoma cells .
Recombinant NOV protein is produced using various expression systems, including HEK and Flp-In-293 cells. The protein is purified using established protocols, and its identity is confirmed through mass spectrometry. The biological activity of the purified protein is demonstrated using assays such as the Smad3-sensitive reporter gene and BrdU proliferation assay .
