M CSF Rat HEK
Description
MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.
Product Specs
Recombinant Rat MCSF, produced in HEK-293 cells, is a secreted protein encompassing amino acids Glu33 to Arg254. This protein exists as a disulfide-linked homodimer, with each subunit containing 222 amino acids.
Lyophilized MCSF demonstrates stability at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at temperatures below -18°C. Once reconstituted, MCSF should be stored at 4°C for 2-7 days. For extended storage, freezing at -18°C is recommended. To enhance long-term stability during storage, consider adding a carrier protein like 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Product Science Overview
M-CSF is a glycoprotein that exists as a homodimer. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The recombinant version of M-CSF, specifically the rat recombinant M-CSF expressed in Human Embryonic Kidney (HEK) cells, is engineered to mimic the natural protein’s structure and function. This recombinant form is used extensively in research and therapeutic applications due to its high purity and biological activity.
- Proliferation and Differentiation: M-CSF stimulates the proliferation and differentiation of monocyte/macrophage lineage cells. This is crucial for maintaining the body’s defense mechanisms against infections and diseases.
- Immune Response: It enhances antibody-dependent cell-mediated cytotoxicity by monocytes and macrophages, thereby boosting the immune response.
- Bone Resorption: M-CSF inhibits bone resorption by osteoclasts, playing a role in bone metabolism and health.
- Atherosclerosis: It is implicated in the development of atherosclerosis, a condition characterized by the buildup of fats, cholesterol, and other substances in and on the artery walls.
The recombinant M-CSF expressed in HEK cells is widely used in various research and clinical settings:
- Cell Culture: It is used to stimulate the growth and differentiation of monocytes and macrophages in vitro.
- Functional Assays: Researchers use M-CSF in functional assays to study its effects on cell proliferation, differentiation, and survival.
- Disease Models: It is used in disease models to understand the role of M-CSF in conditions like atherosclerosis, bone diseases, and immune disorders.
