M CSF Mouse
Macrophage-Colony Stimulating Factor Mouse Recombinant
Cat. No.
BT1892
Source
Escherichia Coli.
Synonyms
CSF-1, Lanimostim, MCSF, M-CSF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.
MCSF is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Granulocyte/Macrophage Colony-Stimulating Factors (GM-CSFs) are cytokines that play a crucial role in hematopoiesis, regulating the production, differentiation, and function of granulocytes and monocytes-macrophages, two related white blood cell populations. Specifically, CSF-1 stimulates the production of cells belonging to the monocyte/macrophage lineage. This cytokine contributes to various physiological processes, including immune responses, bone metabolism, lipoprotein clearance, fertility, and pregnancy.
Description
Recombinant Mouse Macrophage Colony Stimulating Factor (M-CSF), produced in E. coli, is a non-glycosylated homodimer linked by a disulfide bond. The polypeptide chain consists of two units, each containing 156 amino acids, resulting in a total molecular mass of 36.4 kDa. M-CSF undergoes purification using proprietary chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder that has been sterilized by filtration.
Formulation
The protein was subjected to lyophilization from a sterile (0.2 µm filtered) solution with a pH of 7.5, containing 10 mM sodium phosphate and 50 mM sodium chloride.
Solubility
To reconstitute the lyophilized M-CSF, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized M-CSF is stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated state below -18°C. Once reconstituted, M-CSF should be stored at 4°C and used within 2-7 days. For future use, store the reconstituted M-CSF below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity of the M-CSF is determined to be greater than 95.0% using SDS-PAGE analysis.
Biological Activity
The biological activity of M-CSF is determined by its ability to stimulate the proliferation of murine M-NFS-60 indicator cells. The ED50, which represents the concentration of M-CSF required to achieve half-maximal stimulation, is 1.33 ng/ml. This corresponds to a specific activity of 7.5 x 105 units/mg.
Synonyms
CSF-1, Lanimostim, MCSF, M-CSF.
Source
Escherichia Coli.
Amino Acid Sequence
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Product Science Overview
Structure and Function
Recombinant M-CSF
Recombinant M-CSF is produced using recombinant DNA technology, which involves inserting the gene encoding M-CSF into a suitable expression system, such as E. coli or mammalian cells, to produce the protein in large quantities . The recombinant form of M-CSF is used extensively in research to study its biological functions and potential therapeutic applications.
Biological Activity
The biological activity of recombinant M-CSF is determined through various assays, such as the proliferation of M-NFS-60 cells . The activity is measured in units per milligram (U/mg), and the specific activity can vary depending on the production batch . Recombinant M-CSF is used in cell culture, differentiation studies, and functional assays to understand its role in macrophage biology and its potential therapeutic applications .
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