M CSF Human
Greater than 95.0% as determined by SDS-PAGE.
Description
Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 37.1 KD. MCSF is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Human Macrophage Colony Stimulating Factor, produced in E. coli, is a non-glycosylated, disulfide-linked homodimer. Each polypeptide chain consists of 159 amino acids, resulting in a total molecular mass of 37.1 kDa. The purification process of MCSF involves proprietary chromatographic techniques.
The purity of the MCSF is determined by SDS-PAGE analysis and is found to be greater than 95.0%.
The biological activity of MCSF is determined by its ability to stimulate the proliferation of murine M-NFS-60 indicator cells. The ED50 value, which represents the concentration of MCSF required to achieve half-maximal stimulation, is 1.15 ng/ml. This corresponds to a specific activity of 8.7 x 105 Units/mg.
Product Science Overview
M-CSF is a four α-helical bundle cytokine . It is involved in diverse biological processes, including the regulation of inflammatory responses, bone resorption, atherosclerosis, and brain and placental development . The primary function of M-CSF is to regulate the proliferation, differentiation, and survival of monocytes and macrophages . Additionally, M-CSF plays a significant role in immunological defense, bone metabolism, fertility, and pregnancy .
Recombinant human M-CSF is produced using various expression systems, including E. coli and human 293 cells . The recombinant form is used extensively in research to study the differentiation of macrophages from peripheral blood monocytes and the differentiation of osteoclasts from CD14+ monocytes . The recombinant protein is also used in survival studies and apoptosis assays .
Human recombinant M-CSF has several applications in scientific research, including:
- Survival studies and apoptosis assays: For example, using peripheral blood monocytes .
- Differentiation studies: Differentiation of macrophages from peripheral blood monocytes and differentiation of osteoclasts from CD14+ monocytes .
- Functional assays: Used in various cell culture and functional assays to study the biological activity of M-CSF .
The biological activity of recombinant human M-CSF is determined by its ability to regulate the proliferation of NFS-60 cells . The activity is measured in International Units (IU) per milligram, with research-grade M-CSF having an activity of ≥ 1×10^7 IU/mg and premium-grade M-CSF having an activity of ≥ 2×10^7 IU/mg .
