MCSFR Human
Sf9, Baculovirus cells.
Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor (EC:2.7.10.1), CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1R, FMS.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
MCSFR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 737 amino acids (20-517a.a.) and having a molecular mass of 82.1kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
MCSFR is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The Macrophage Colony-Stimulating Factor Receptor (MCSFR or CSF1R) belongs to the type 3 subfamily of receptor tyrosine kinases. Its expression is primarily observed in cells of monocyte and macrophage lineages, stem cells, and the developing placenta. MCSFR plays a crucial role in mediating the biological effects of the cytokine CSF1. Structurally, it consists of an extracellular ligand-binding domain, a single transmembrane segment, and an intracellular tyrosine kinase domain. The CSF1/CSF1R axis has been identified as essential for both normal trophoblastic implantation and monocyte development. Interestingly, this axis has also been implicated in normal mammary gland development and its dysregulation has been linked to breast cancer. Elevated levels of CSF1R have been observed in microglia in the context of Alzheimer's disease and brain injuries, leading to increased microglial activity.
MCSFR, expressed in Sf9 insect cells using a baculovirus system, is produced as a single, glycosylated polypeptide chain. It comprises 737 amino acids, with a sequence spanning from residue 20 to 517, and has a molecular weight of 82.1 kDa. On SDS-PAGE, the apparent molecular size is expected to range from 70 kDa to 100 kDa. The protein includes a 239 amino acid hIgG-His tag located at the C-terminus and is purified using proprietary chromatographic methods.
The product appears as a clear, colorless solution after sterile filtration.
The MCSFR protein solution is provided at a concentration of 0.25 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. Repeated freeze-thaw cycles should be minimized to maintain protein integrity.
SDS-PAGE analysis indicates a purity level exceeding 90.0%.
The biological activity of MCSFR is assessed based on its ability to inhibit the proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells in a manner dependent on Macrophage Colony-Stimulating Factor (M-CSF). The ED50 value, representing the concentration at which 50% inhibition is observed, is determined to be less than or equal to 100 ng/ml in the presence of 10 ng/ml M-CSF.
Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor (EC:2.7.10.1), CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1R, FMS.
Sf9, Baculovirus cells.
IPVIEPSVPE LVVKPGATVT LRCVGNGSVE WDGPPSPHWT LYSDGSSSIL STNNATFQNT GTYRCTEPGD PLGGSAAIHL YVKDPARPWN VLAQEVVVFE DQDALLPCLL TDPVLEAGVS LVRVRGRPLM RHTNYSFSPW HGFTIHRAKF IQSQDYQCSA LMGGRKVMSI SIRLKVQKVI PGPPALTLVP AELVRIRGEA AQIVCSASSV DVNFDVFLQH NNTKLAIPQQ SDFHNNRYQK VLTLNLDQVD FQHAGNYSCV ASNVQGKHST SMFFRVVESA YLNLSSEQNL IQEVTVGEGL NLKVMVEAYP GLQGFNWTYL GPFSDHQPEP KLANATTKDT YRHTFTLSLP RLKPSEAGRY SFLARNPGGW RALTFELTLR YPPEVSVIWT FINGSGTLLC AASGYPQPNV TWLQCSGHTD RCDEAQVLQV WDDPYPEVLS QEPFHKVTVQ SLLTVETLEH NQTYECRAHN SVGSGSWAFI PISAGAHTHP PDEFLFTPLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.
Product Science Overview
In the human genome, the CSF1R gene is located on chromosome 5 (5q32) . The gene is 60.002 kilobases in length and is highly expressed in myeloid cells . Hematopoietic stem cells express CSF1R at low levels, but it is highly expressed in more differentiated myeloid cell types such as monocytes, macrophages, osteoclasts, myeloid dendritic cells, microglia, and Paneth cells .
CSF1R can be activated by two ligands: colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34) . Activation of CSF1R signaling is crucial for the survival, proliferation, and differentiation of many myeloid cell types both in vivo and in vitro . The receptor’s signaling pathway is involved in various physiological processes and diseases, making it a target for therapies in cancer, neurodegeneration, and inflammatory bone diseases .
The expression of CSF1R is controlled by two alternative promoters that are active in specific tissue types . Exon 1 of CSF1R is specifically transcribed in trophoblastic cells, while exon 2 is transcribed in macrophages . The activation of CSF1R transcription is regulated by several transcription factors, including Ets and PU.1 . Additionally, a highly conserved region termed the fms intronic regulatory element (FIRE) regulates transcript elongation during transcription of CSF1R in macrophages .
CSF1R signaling is implicated in many diseases and is targeted in therapies for cancer, neurodegeneration, and inflammatory bone diseases . The receptor plays a significant role in regulating the survival, proliferation, and differentiation of myeloid cells, particularly the macrophage lineage . Loss of CSF1R in mice results in postnatal morbidity and lethality due to an osteopetrotic phenotype, highlighting its critical role in bone formation and hematopoiesis .
Research has shown that CSF1R is a major regulator of microglial development and maintenance in the brain . Additionally, studies have identified CSF1 as an extrinsic stimulator of spermatogonial stem cell self-renewal, implicating Leydig and myoid cells as contributors to the testicular stem cell niche in mammals .
