MCSF Mouse, Sf9
M-Csf, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, C87615, MCSF, op, Processedmacrophage colony-stimulating factor 1.
Greater than 90.0% as determined by SDS-PAGE.
Description
MCSF Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 164 amino acids (33-187 aa) and having a molecular mass of 19.1 kDa.
MCSF is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Macrophage Colony Stimulating Factor (M CSF), also known as colony stimulating factor 1, is a cytokine that promotes the differentiation of hematopoietic stem cells into macrophages. Additionally, M CSF binds to its receptor (colony stimulating factor 1 receptor) and plays a role in placenta growth and development.
Produced in Sf9 Baculovirus cells, MCSF Mouse is a single, glycosylated polypeptide chain consisting of 164 amino acids (33-187 aa). It has a molecular mass of 19.1 kDa. The protein includes a 6 amino acid His tag fused at the C-terminus and is purified using proprietary chromatographic techniques.
The MCSF solution has a concentration of 1mg/ml and contains 10% Glycerol and Phosphate-Buffered Saline with a pH of 7.4.
For optimal storage, keep the vial at 4°C if it will be used within 2-4 weeks. For longer periods, store the solution frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. It's important to avoid subjecting the solution to multiple freeze-thaw cycles.
The purity of the MCSF Mouse is determined by SDS-PAGE to be greater than 90.0%.
The biological activity is assessed through a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range for this effect is approximately 4ng/ml.
M-Csf, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, C87615, MCSF, op, Processedmacrophage colony-stimulating factor 1.
ADPKEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV
QDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI
KNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKPHH HHHH
Product Science Overview
M-CSF is a homodimeric glycoprotein growth factor that regulates the proliferation and differentiation of myeloid hematopoietic progenitors to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts . The mouse recombinant form of M-CSF is produced using the Sf9 insect cell line, which is derived from the fall armyworm (Spodoptera frugiperda). This production method ensures high purity and biological activity of the recombinant protein.
M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase known as CSF-1R (colony-stimulating factor 1 receptor), which is encoded by the c-fms proto-oncogene . Upon binding to M-CSF, CSF-1R activates several downstream signaling pathways, including MAPK, PI3K, and PLCγ . These pathways are crucial for the regulation of macrophage proliferation, differentiation, and survival.
M-CSF plays a vital role in the development and maintenance of macrophages, key immune cells involved in innate immunity and tissue homeostasis . It is required for the maturation and activation of monocytes and macrophages and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 . Additionally, M-CSF is involved in bone resorption by osteoclasts and plays a role in the development and regulation of the placenta, mammary gland, and brain .
The modulation of M-CSF has therapeutic potential in various diseases. For example, targeting M-CSF signaling pathways can be beneficial in treating inflammatory diseases, bone disorders, and certain cancers . Research is ongoing to explore the full therapeutic potential of M-CSF modulation in clinical settings.
