CD Antigens
Product List
CD200 Human, Sf9
CD200 Human Recombinant, sf9
CD200 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 444 amino acids (31-232a.a.) and having a molecular mass of 49.7kDa (Molecular size on SDS-PAGE will appear at approximately 57-70 kDa).
CD200 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
CD200 Human
CD200 Human Recombinant
CD200 is fused to a 23 amino acid His-tag at N-terminus.
CD200 Mouse
CD200 Mouse Recombinant
CD200 Mouse Recombinant produced in Baculovirus is a single, glycosylated, polypeptide chain containing 441 amino acids (31-232aa) and having a molecular mass of 49.5kDa.
CD200 is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
CD200R1 Human
CD200 Receptor 1 Human Recombinant
CD200R1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 462 amino acids (24-243 a.a.) and having a molecular mass of 51.9kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CD200R1 is expressed with 242 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Sterile filtered colorless solution.
CD200R1 Mouse
CD200 Receptor 1 Mouse Recombinant
CD200R1 is expressed with a 239 amino acid hIgG His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction
CD200, also known as OX-2, is a type-1 membrane glycoprotein that belongs to the immunoglobulin superfamily (IgSF). It is encoded by the CD200 gene located on chromosome 3q13.2 in humans . CD200 is characterized by two extracellular immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail .
Key Biological Properties: CD200 is involved in immunosuppressive signaling through its interaction with the CD200 receptor (CD200R) .
Expression Patterns and Tissue Distribution: CD200 is broadly expressed on various cell types, including B lymphocytes, a subset of T lymphocytes, dendritic cells, endothelial cells, and neurons . It is also found in tissues such as the frontal pole, hypothalamus, and aorta .
Primary Biological Functions: CD200 plays a crucial role in regulating immune responses by delivering immunosuppressive signals through its receptor, CD200R . This interaction helps maintain immune homeostasis and promotes self-tolerance .
Role in Immune Responses and Pathogen Recognition: CD200 modulates immune cell activity by inhibiting proinflammatory cytokine secretion, enhancing anti-inflammatory cytokine secretion, and promoting the induction of regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) . It also inhibits natural killer (NK) cell activation and cytotoxic T cell functions .
Mechanisms with Other Molecules and Cells: CD200 interacts with its receptor, CD200R, which is expressed on monocytes, myeloid cells, and T lymphocytes . The engagement of CD200 with CD200R leads to tyrosine phosphorylation on the CD200R cytoplasmic PTB domain, recruiting adaptor proteins DOK-1 and DOK-2 .
Binding Partners and Downstream Signaling Cascades: The binding of CD200 to CD200R promotes the binding of SHIP to DOK-1 and the recruitment of RasGAP, which negatively regulates the MAPK/ERK signaling pathway . This signaling cascade results in the inhibition of immune responses and the promotion of immune tolerance .
Regulatory Mechanisms Controlling Expression and Activity: The expression of CD200 is regulated at the transcriptional level by C/EBP-β, which also regulates responses induced by IFN-γ, IL-6, IL-1, and TNF-α .
Transcriptional Regulation and Post-Translational Modifications: CD200 expression is modulated by various cytokines and transcription factors, ensuring its appropriate expression in different tissues and under different physiological conditions .
Biomedical Research: CD200 is used as a marker in the differential diagnosis of B-cell neoplasms and in monitoring minimal residual disease .
Diagnostic Tools: Anti-CD200 antibodies are employed in flow cytometry, immunohistochemistry, Western blot, ELISA, and immunocytochemistry for detecting CD200 expression .
Therapeutic Strategies: Targeting the CD200/CD200R axis has shown potential in cancer immunotherapy, particularly in treating hematologic malignancies and solid tumors . CD200 checkpoint reversal is being explored as a novel approach to immunotherapy .
Role Throughout the Life Cycle: CD200 plays a role in various stages of life, from development to aging and disease. It contributes to immune regulation during development and helps maintain immune homeostasis in adulthood . In aging and disease, CD200 expression patterns can influence the progression of conditions such as chronic lymphocytic leukemia and other malignancies .
CD200’s role in enhancing synaptic plasticity and promoting functional recovery after stroke further highlights its importance in neurological health .
