CD Antigens
Product List
CD300A Human
CD300A Human Recombinant
CD300C Human
CD300C Human Recombinant
CD300C Human, Sf9
CD300C Human Recombinant, Sf9
CD300C Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 163 amino acids (29-183a.a.) and having a molecular mass of 18kDa (Molecular size on SDS-PAGE will appear at approximately 28-40 kDa). CD300C is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
CD300E Human
CD300E Human Recombinant
CD300E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
The CD300 family of molecules, also known as the CMRF-35-like molecules (CLM) in humans, are a group of cell surface receptors that play crucial roles in the regulation of immune responses. These receptors are classified into activating and inhibitory types based on their functions and signaling motifs. The human CD300 family consists of seven members located on chromosome 17, named alphabetically from CD300a to CD300g .
Key Biological Properties: CD300 molecules are characterized by their ability to recognize and bind to lipids such as phosphatidylserine and phosphatidylethanolamine, which are exposed on the outer leaflet of the plasma membrane of dead and activated cells .
Expression Patterns and Tissue Distribution: CD300 receptors are broadly expressed on various immune cells, including myeloid and lymphoid cells. Their expression is differentially regulated depending on the cell type and the stimuli they encounter .
Primary Biological Functions: CD300 receptors modulate a wide array of immune cell processes through their paired activating and inhibitory receptor functions. They play a significant role in fine-tuning immune responses and maintaining immune homeostasis .
Role in Immune Responses and Pathogen Recognition: CD300 molecules are involved in the recognition of pathogens and the regulation of immune responses. For instance, CD300a, an inhibitory receptor, recognizes phosphatidylserine and phosphatidylethanolamine on dead and activated cells, modulating immune functions and participating in the host response to various diseases .
Mechanisms with Other Molecules and Cells: CD300 receptors interact with other molecules and cells through their extracellular IgV-like domains. Activating members have a short intracellular tail and associate with ITAM-containing adaptor proteins, such as DAP12 and FcεRIγ, while inhibitory receptors have a long intracellular tail carrying ITIMs .
Binding Partners and Downstream Signaling Cascades: The binding of CD300 receptors to their ligands triggers downstream signaling cascades that either activate or inhibit immune cell functions. For example, the inhibitory signal of CD300a depends on the phosphorylation of tyrosine residues embedded in ITIMs of its cytoplasmic tail .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CD300 receptors are regulated by various stimuli. For instance, in CD4+ T cells, stimulation with anti-CD3 plus anti-CD28 mAbs and Th1-differentiation conditions upregulate CD300a expression, while TGF-β exhibits a negative regulatory effect .
Transcriptional Regulation and Post-Translational Modifications: The transcriptional regulation of CD300 genes involves multiple factors, and post-translational modifications such as phosphorylation play a crucial role in modulating their activity .
Biomedical Research: CD300 receptors are valuable tools in biomedical research for understanding immune regulation and identifying potential therapeutic targets .
Diagnostic Tools and Therapeutic Strategies: CD300 molecules have potential applications as diagnostic markers and therapeutic targets in various diseases, including infectious diseases, allergies, cancer, and chronic inflammatory conditions .
Role Throughout the Life Cycle: CD300 receptors play essential roles throughout the life cycle, from development to aging and disease. They are involved in the regulation of immune responses during development, maintaining immune homeostasis in adulthood, and modulating immune functions in aging and disease states .
