CD300C Human, Sf9

CD300C, also known as CMRF35-like molecule 6 (CLM-6), is a member of the CD300 family of receptors. These receptors play a crucial role in modulating immune cell processes through their paired activating and inhibitory functions. CD300C is expressed on various immune cells, including monocytes, neutrophils, and some T and B lymphocytes .

CD300C is a single, glycosylated polypeptide chain containing 163 amino acids (29-183a.a.) and has a molecular mass of approximately 18kDa. When analyzed using SDS-PAGE, the molecular size appears to be between 28-40 kDa . The recombinant form of CD300C is produced in Sf9 Baculovirus cells, which is a common method for expressing recombinant proteins due to its efficiency and ability to produce glycosylated proteins .

The CD300 family of receptors, including CD300C, is involved in recognizing lipids such as extracellular ceramide, phosphatidylserine, and phosphatidylethanolamine. These lipids are exposed on the outer leaflet of the plasma membrane of dead and activated cells. By binding to these lipids and other ligands, CD300C and its family members play significant roles in various biological processes and the host response to pathological conditions .

CD300C and other CD300 family members have been implicated in the pathogenesis of several diseases. They hold potential as targets for diagnostic and therapeutic purposes in conditions such as infectious diseases, allergies, and cancer. The ability of CD300C to modulate immune responses makes it a valuable target for research and potential clinical applications .

The recombinant CD300C protein produced in Sf9 Baculovirus cells is purified using proprietary chromatographic techniques. It is typically supplied as a sterile, filtered colorless solution containing phosphate-buffered saline (pH 7.4) and 10% glycerol. The protein is stable when stored at 4°C for short-term use and at -20°C for long-term storage. It is recommended to add a carrier protein to prevent multiple freeze-thaw cycles .