CD300C Human

CD300C is a type I transmembrane protein that contains:

• An IgV-like extracellular domain with two disulfide bonds.
• A proline-rich membrane proximal region.
• A transmembrane region with a charged glutamic acid.
• A cytoplasmic tail of 18 amino acids .

The human CD300 family has seven members, while the mouse family has nine. The genes encoding these receptors are located on chromosome 17 in humans and on the syntenic region of chromosome 11 in mice .

CD300C is primarily expressed on various immune cells, including B cells, dendritic cells, macrophages, and monocytes . It plays a significant role in regulating immune responses by:

• Inhibiting the proliferation of activated T cells.
• Modulating cytokine production, including IL-2, IFN-γ, TNF-α, and IL-17A .
• Ameliorating graft-versus-host disease (GvHD) in experimental models .

In natural killer (NK) cells, crosslinking CD300C with an antibody induces cytokine secretion and degranulation, highlighting its role in immune cell activation .

Recombinant human CD300C is produced using various expression systems, such as HEK293 cells. The recombinant protein typically includes a polyhistidine tag for purification and detection purposes . It is used in research to study the function of CD300C and its role in immune regulation.

The recombinant protein is characterized by:

• A predicted molecular mass of approximately 18.4 kDa, though it appears as 40-45 kDa in SDS-PAGE due to glycosylation .
• High purity, typically greater than 95%, as determined by SDS-PAGE .
• Low endotoxin levels, ensuring its suitability for in vitro and in vivo studies .

The CD300 family, including CD300C, is emerging as an important group of regulators in the immune system. Understanding the function and regulation of these receptors can provide insights into immune-related diseases and potential therapeutic targets .